Novel peptide-forming enzyme gene
AssigneeAjinomoto Co., Inc.
InventorsSeiichi HARA, Kenzo Yokozeki, Isao Abe, Naoto Tonouchi, Yasuko Jojima
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Abstract
DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.
Claims31
An isolated DNA encoding a protein selected from the group consisting of (A) and (M), wherein said protein has an amino acid sequence defined as follows:
- (A) an amino acid sequence consisting of amino acid residue numbers 23 to 616 of SEQ ID NO:6, and
- (M) an amino acid sequence consisting of SEQ ID NO:6.
An isolated DNA encoding a protein selected from the group consisting of (B) and (N), wherein said protein has an amino acid sequence defined as follows:
- (B) an amino acid sequence including substitution, deletion, insertion, and/or addition, of one to 30 amino acids in amino acid residue numbers 23 to 616 of SEQ ID NO:6, and has at least 50% of the peptide-forming activity of a protein corresponding to unmutated amino acid residue numbers 23 to 616 of SEQ ID NO:6 at 50° C. and a pH of 8, and
- (N) an amino acid sequence including substitution, deletion, insertion, and/or addition, of one to 30 amino acids in an amino acid sequence consisting of SEQ ID NO:6, and has at least 50% of the peptide-forming activity of a protein corresponding to unmutated SEQ ID NO:6 at 50° C. and a pH of 8.
An isolated DNA selected from the group consisting of (a) and (m), wherein said DNA has a nucleotide sequence defined as follows:
- (a) a nucleotide sequence consisting of nucleotide numbers 127 to 1908 of SEQ ID NO:5, and
- (m) a nucleotide sequence consisting of nucleotide numbers 61 to 1908 of SEQ ID NO:5.
Dependencies are inferred from the claim text.