Microbial Growth Assay

An assay device comprising:

• a cell culture device comprising a plurality of wells, each well comprising a substantially planar bottom and at least one wall;
• a plurality of supports, each support disposed within a well perpendicular to the substantially planar bottom, wherein the plurality of supports comprise discs;
• and
• at least one cover that fittably seals the top of each well.

An assay system comprising:

• a cell culture device comprising a plurality of wells and/or channels, each well or channel comprising a substantially planar bottom;
• a plurality of supports, each support disposed within a well or channel perpendicular to the substantially planar bottom;
• and
• at least one cover that fittably seals the top of each well or channel;
• culturing a sample comprising a microbial population in a media within the wells or channels such that the media is in contact with the supports thereby forming a biofilm on the supports;
• measuring a fluorometric or calorimetric absorbance from a fluorogenic or chromogenic moiety in the sample;
• comparing the fluorogenic or calorimetric absorbance to a standard sample.