Method of Analyzing Dynamics in the Differentiation of Vascular Endothelial Progenitor Cells
OwnerTakayuki Asahara, Haruchika Masudainventors
Life of this patent
Estimating the term…
Loading patent…
OwnerTakayuki Asahara, Haruchika Masudainventors
Estimating the term…
The present invention provides a method of forming an EPC colony with good reproducibility, and a method of analyzing the dynamics of EPC differentiation in the body of patient. More specifically, the present invention provides a method of analyzing the dynamics of differentiation of endothelial progenitor cells, which includes culturing hemangioblasts in a semisolid medium containing a vascular endothelial growth factor and a basic fibroblast growth factor, and evaluating the mode of endothelial progenitor cell colony formation; a method of forming an endothelial progenitor cell colony which includes culturing hemangioblasts in a semisolid medium containing a vascular endothelial growth factor and a basic fibroblast growth factor; a semisolid medium containing a vascular endothelial growth factor and a basic fibroblast growth factor; and a kit for preparing the semisolid medium and the like.
A method of analyzing dynamics of differentiation of an endothelial progenitor cell, which comprises culturing a hemangioblast in a semisolid medium containing a vascular endothelial growth factor and a basic fibroblast growth factor, and evaluating the mode of vascular endothelial progenitor cell colony formation.
A method of forming an endothelial progenitor cell colony, which comprises culturing a hemangioblast in a semisolid medium containing a vascular endothelial growth factor and a basic fibroblast growth factor and confirming the appearance of an endothelial progenitor cell colony.
A semisolid medium containing a vascular endothelial growth factor and a basic fibroblast growth factor.
A kit for preparing a semisolid medium containing a vascular endothelial growth factor and a basic fibroblast growth factor, which comprises a vascular endothelial growth factor, a basic fibroblast growth factor and a semisolid medium (one or both of the vascular endothelial growth factor and the basic fibroblast growth factor has/have not been added to the semisolid medium).
Dependencies are inferred from the claim text.