Device and method for measuring LDL-associated cholesterol
AssigneeCholestech Corporation
InventorsShireen Khan, Suyue Qian, Jeff Shindelman, George E. Withers, Eileen Gee, William H. Chapman, Greg Bennett, Thomas D. Schaal
Life of this patent
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Abstract
An assay device and method for determining the concentration of low density lipoprotein (LDL)-associated cholesterol (LDL-C) in a body-fluid sample, such as a blood sample is described.
Claims22
An assay device for determining the amount of LDL-associated cholesterol in a blood-fluid sample containing very low density lipoprotein (VLDL), chylomicrons, low-density lipoprotein (LDL), high-density lipoproteins (HDL), and triglycerides, comprising:
- (a) a body having a sample well for receiving such a sample;
- (b) first and second detection zones mounted on said body for producing a detectable product related to the amount of HDL and an adjusted total cholesterol composed of HDL plus LDL, respectively, in said sample fluid,
- (c) fluid flow pathways for transferring sample fluid from the sample well to said detection zones, and
- (d) reagents in one or more of said flowpaths, and optionally, in said sample well, for selectively removing VLDL and chylomicrons in sample fluid transferred from the sample well to the first and second detection zones, and for selectively removing LDL in sample fluid transferred from the sample well to the second detection zone.
A method for determining the amount of LDL-associated cholesterol in a blood-fluid sample containing very low density lipoprotein (VLDL), chylomicrons, low-density lipoprotein (LDL), high-density lipoproteins (HDL), and triglycerides, comprising:
- (a) measuring the levels of HDL and an adjusted total cholesterol composed of HDL plus LDL, respectively, in such a sample fluid, and
- (b) determining sample LDL level based on the difference between total adjusted cholesterol and HDL.
Dependencies are inferred from the claim text.