Method and device for monitoring inhibition of platelet function
OwnerDaniel G. Ericsoninventor
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Abstract
The invention provides a method of monitoring the response of platelets to a COX1 inhibitor such as aspirin. The method involves collecting platelet-containing mammalian blood treated with a COX1 inhibitor; mixing the blood with a COX1-dependent platelet agonist, such as arachidonic acid, monitoring extracellular ATP in the agonist-activated blood to generate a measurement, and comparing the measurement to a standard value. Devices, systems, and kits for carrying out the method are also provided.
Claims20
A method of monitoring inhibition of platelet function comprising:
- collecting platelet-containing mammalian blood treated with a COX inhibitor;
- mixing the blood with a platelet agonist to generate agonist-activated blood;
- monitoring an extracellular platelet-release-reaction product in the agonist-activated blood to generate a measurement;
- and
- comparing the measurement to a standard value.
A device for monitoring COX1 inhibitor response comprising:
- a fluid-tight material forming an assay chamber;
- a pump functionally linked to the assay chamber for pumping fluids into the assay chamber;
- a light detector functionally linked to the assay chamber for detecting light emitted in the assay chamber;
- a fluid-tight material forming a blood chamber for holding blood, the blood chamber functionally linked to the pump;
- a fluid-tight material forming an enzyme chamber for holding a solution of a light-producing ATP-consuming enzyme, the enzyme chamber functionally linked to the pump;
- and a fluid-tight material forming an agonist chamber for holding a solution of a COX1-dependent platelet agonist, the agonist chamber functionally linked to the pump.
A system for monitoring COX1-inhibitor response comprising:
- a fluid-tight material forming an assay chamber;
- a pump functionally linked to the assay chamber for pumping fluids into the assay chamber;
- a light detector functionally linked to the assay chamber for detecting light emitted in the assay chamber;
- a fluid-tight material forming a blood chamber for holding blood, the blood chamber functionally linked to the pump;
- a fluid-tight material forming an enzyme chamber for holding a solution of a light-producing ATP-consuming enzyme, the enzyme chamber functionally linked to the pump;
- a fluid-tight material forming an agonist chamber for holding a solution of a COX1-dependent platelet agonist, the agonist chamber functionally linked to the pump;
- a controller coupled to the pump and programmed to deliver to the assay chamber a predetermined volume of the blood, a predetermined volume of the light-producing enzyme solution, and a predetermined volume of the agonist solution;
- and a display operably coupled to the light detector for displaying results from the light detector.
Dependencies are inferred from the claim text.