Assays for TERT promoter modulatory agents
OwnerWilliam H. Andrews, Laura A. Briggs +3inventors
InventorsWilliam H. Andrews, Laura A. Briggs, Christopher A. Foster, Mieczyslaw A. Piatyszek, Lancer K. Brown
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Abstract
Methods and compositions for assaying an agent for TERT promoter modulatory activity are provided. In the subject methods, an agent is contacted with a cell comprising a mutant telomerase structural RNA component (TR) that results in a detectable phenotype in the presence of telomerase reverse transcriptase (TERT). Also provided are compositions, systems and kits thereof, as well as devices, that find use in practicing the subject methods. The subject invention finds use in assaying agents for TERT promoter modulatory activity, such as in a high throughput format.
Claims24
A method of determining whether an agent modulates transcription control activity of a TERT promoter, said method comprising:
- (a) contacting said agent with a cell comprising a mutant telomerase structural RNA component (TR) that results in a detectable phenotype in the presence of telomerase reverse transcriptase (TERT);
- and
- (b) evaluating said cell for said detectable phenotype to determine whether said agent modulates transcription control activity of said TERT promoter nucleic acid.
A method of determining whether a small molecule agent can derepress transcription repression activity of a TERT promoter, said method comprising:
- (a) contacting said agent with a cell comprising a mutant telomerase structural RNA component (TR) that results in a detectable phenotype in the presence of telomerase reverse transcriptase (TERT);
- and
- (b) evaluating said cell for said detectable phenotype to determine whether said agent derepresses transcription repression activity of said TERT promoter nucleic acid.
Dependencies are inferred from the claim text.