Detection of nucleic acid associated with disease

A method for detecting the presence of a first nucleic acid of a pathogenic microorganism present in said individual and a second nucleic acid of an individual subject to a specific disease, wherein said pathogenic organism is associated with the specific disease comprising,

• (a) extracting a first sample nucleic acid of the pathogenic microorganism and a second sample nucleic acid of the individual from a specimen sampled from said individual without isolating the first and second sample nucleic acids;
• (b) reacting the extract of first and second sample nucleic acids with a probe-immobilized substrate comprising a first probe and a second probe, wherein said first probe detects the presence of a specific nucleic acid sequence of the pathogenic microorganism, wherein said pathogenic microorganism is associated with said specific disease, and wherein said second probe detects the presence of a specific nucleic acid sequence of said individual;
• and
• (c) detecting the presence of said first nucleic acid by detecting the presence of nucleic acid bound to said first probe, and detecting the presence of nucleic acid bound to said second probe, resulting from said reaction of (b).