Method for Detecting ncRna
OwnerJian Haninventor
Life of this patent
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Abstract
Described is a novel method for the detection of ncRNA molecules. The disclosed method is especially useful for the detection miRNA and siRNA. The method can be used to generate a profile of the ncRNA molecules present in a sample. In addition, using the methods of the present disclosure a ncRNA signature for a given disease or condition can be created. The ncRNA signature can be used for diagnostic purposes, therapeutic purposes and drug discovery purposes, as well as other uses.
Claims82
A method for the simultaneous detection of a plurality of distinct target ncRNAs, said method comprising the steps of:
- a. providing a RNA sample from a subject, said sample containing said plurality of target ncRNAs;
- b. contacting said sample with a first oligonucleotide specific for each of said target ncRNAs to be detected under conditions appropriate to form a complex between said first oligonucleotides and said target ncRNAs, each of said first oligonucleotides comprising a first signal generator to generate a first detectable signal and each of said first oligonucleotides having a first Tm for binding each of said target ncRNAs that is substantially the same;
- c. contacting said sample with a second oligonucleotide to bind for each of said target RNA to be detected under conditions appropriate to form a complex between said second oligonucleotides and said target ncRNAs, said second oligonucleotide comprising a second signal generator to generate a second detectable signal and each of said second oligonucleotides having a second Tm for binding each of said target ncRNAs that is substantially the same;
- and
- d. determining the presence of said plurality of target ncRNA in said sample by measuring the first and second detectable signals.
A method for determining a profile of a plurality of target ncRNA molecules in a RNA sample, said method comprising the steps of:
- a. providing said RNA sample from a subject, said sample containing said plurality of target ncRNAs;
- b. contacting said sample with a first oligonucleotide specific for each of said target ncRNAs to be detected under conditions appropriate to form a complex between said first oligonucleotides and said target ncRNAs, each of said first oligonucleotides comprising a first signal generator to generate a first detectable signal and each of said first oligonucleotides having a first Tm for binding each of said target ncRNAs that is substantially the same;
- c. contacting said sample with a second oligonucleotide to bind for each of said target RNA to be detected under conditions appropriate to form a complex between said second oligonucleotides and said target ncRNAs, said second oligonucleotide comprising a second signal generator to generate a second detectable signal and each of said second oligonucleotides having a second Tm for binding each of said target ncRNAs that is substantially the same;
- and
- d. determining the presence of said plurality of target ncRNA in said sample by measuring the first and second detectable signals.
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A method for the detection of a target ncRNA, said method comprising the steps of:
- a. providing a RNA sample from a subject, said sample containing said target ncRNA
- b. contacting said sample with a first oligonucleotide specific for said target RNA under conditions appropriate to form a complex between said first oligonucleotide and said target ncRNA, said first oligonucleotide comprising a first signal generator to generate a first detectable signal;
- c. contacting said sample with a second oligonucleotide specific for said target RNA under conditions appropriate to form a complex between said second oligonucleotide and said target ncRNA, said second oligonucleotide comprising a second signal generator to generate a second detectable signal;
- and
- d. determining the presence of said target ncRNA in said sample by measuring the first and second detectable signals.
Dependencies are inferred from the claim text.