Rapid and Low Cost Method for Isolating Nucleic Acid
AssigneeQiagen GmbH
InventorsRalf Himmelreich, Sabine Werner, Claudia Fritz
Life of this patent
Estimating the term…
Abstract
The invention relates to a rapid method for isolating nucleic acid from a nucleic acid source, wherein the nucleic acid source is lysed in the absence of a chaotropic salt and in the absence of an alcohol. The lysate is subsequently filtered through a porous matrix consisting of a material based on silica or of a silica coated material, which binds the nucleic acid in the absence of a chaotropic salt and in the absence of an alcohol. Finally, the nucleic acid is eluted from the porous matrix by an aqueous buffer solution. Furthermore, this invention relates to a test kit in order to isolate the nucleic acid.
Claims23
A method for rapidly isolating nucleic acid from a nucleic acid source comprising the steps of:
- a) lysing the nucleic acid source,
- b) filtering the lysate through a porous matrix consisting of a material based on silica or of a silica coated material to bind the nucleic acid to the porous matrix in the absence of an alcohol and in the absence of a chaotropic salt,
- c) eluting the nucleic acid from the porous matrix of step b) by using an aqueous buffer solution.
Dependencies are inferred from the claim text.