Method For Detecting Viable Cells In A Sample By Using A Virus
AssigneeEnigma Diagnostics Limited
InventorsDavid James Squirrell, Martin Alan Lee
Life of this patent
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Abstract
A method for detecting viable cells such as bacterial cells, within a sample, said method comprising (i) incubating said sample with a virus which is able to infect said cells under conditions which allow said virus to infect and replicate within any such cells which are viable; (ii) detecting any nucleic acid obtained by replication of the virus in said cell.
Claims20
A method for detecting viable cells within a sample, said method comprising
- (i) incubating said sample with a virus, which is able to infect said cells under conditions which allow said virus to infect and replicate within any such cells which are viable;
- (ii) detecting any nucleic acid obtained by replication of the virus in said cell, provided that when the virus is a modified virus which has been modified to include a marker sequence, the nucleic acid detected is one which is uniquely produced on replication of the virus within the cell.
A kit for carrying out a method for detecting a particular cell according to any one of the preceding claims, said kit comprising a virus, and one or more reagents necessary for detecting nucleic acid obtained by replication of the virus in said cell, provided that when the virus is a modified virus which has been modified to include a marker sequence, the one or more reagents are necessary for detecting a nucleic acid which is uniquely produced on replication of the virus within the cell.
A RNA virus preparation which has been treated to remove contaminating DNA.
Dependencies are inferred from the claim text.