Method to Inhibit the Propagation of an Undesired Cell Population
OwnerCelina Cziepluch, Jean Rommelaere +1inventors
InventorsCelina Cziepluch, Jean Rommelaere, Marc Winnefeld
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Abstract
Disclosed is a method for inhibiting the propagation of an undesired cell population, the method comprising introducing into a cell an antagonist of SGT, or of a functional variant thereof, said antagonist depleting SGT, a functional variant thereof, in a cell of said cell population, and verifying the reduced proliferation and/or cell death in SGT depleted cell populations; disclosed is further the use of a SGT antagonist for the manufacture of a medicament for the treatment of diseases which are caused by the propagation of an undesired cell population.
Claims30
1-29. (canceled)
A method for inhibiting the propagation of an undesired cell population, the method comprising:
- (i) introducing an antagonist of a polypeptide having (i) an amino acid sequence as shown in SEQ ID No:
- 1 or (ii) an amino acid sequence at least 65% identical thereto into at least one cell of an undesired cell population, and
- (ii) cultivating the cell population for a time period sufficient to allow the antagonist to be effective, thereby inactivating, depleting, or inactivating and depleting, the aforementioned polypeptide in the cell population.
A method of treating a disease which is caused by the propagation of an undesired cell population comprising administering to a subject in need a therapeutically effective amount of an antagonist for a polypeptide having (i) an amino acid sequence as shown in SEQ ID No:
- 1 or (ii) an amino acid sequence at least 65% identical thereto.
A composition comprising an antagonist for a polypeptide having (i) an amino acid sequence as shown in SEQ ID No:
- 1 or (ii) an amino acid sequence at least 65% identical thereto, optionally together with a pharmaceutically acceptable carrier, for the treatment of a disease caused by the propagation of an undesired cell population.
A method for screening candidate compounds for at least one antagonist for a polypeptide having (i) an amino acid sequence as shown in SEQ ID No:
- 1 or (ii) an amino acid sequence at least 65% identical thereto with the ability to inhibit the propagation of a cell population, the method comprising the following steps:
- (i) contacting a cell population with a candidate compound, thereby enabling the introduction of the candidate compound into the cells of the cell population,
- (ii) cultivating the cell population for a time period sufficient to allow the candidate compound to be effective, and parallel cultivating a control cell population which has not been contacted with the candidate compound, and
- (iii) monitoring cell growth, cell properties, or a combination thereof in the cell population and in the control cell population,
- wherein a reduced growth, altered cell properties, or a combination thereof as compared to the control cell population is indicative that the candidate compound is an antagonist for the aforementioned polypeptide.
A method for inhibiting the propagation of an undesired cell population, the method comprising
- (i) introducing an antagonist of a polypeptide having an (i) amino acid sequence as shown in SEQ ID No:
- 1 or (ii) an amino acid sequence at least 65% identical thereto into at least one cell of an undesired cell population, and
- (ii) cultivating the cell population for a time period sufficient to allow the antagonist to be effective, thereby inactivating, depleting, or inactivating and depleting the polypeptide in the cell population,
- wherein the antagonist for the aforementioned polypeptide is selected from the group consisting of:
- a) a specific siRNA for a polypeptide having (i) an amino acid sequence as shown in SEQ ID) No:
- 1 or (ii) an amino acid sequence at least 65% identical thereto,
- b) an antisense molecule for the gene encoding a polypeptide having (i) an amino acid sequence as shown in SEQ ID No:
- 1 or (ii) an amino acid sequence at least 65% identical thereto,
- c) a specific ribozyme for the mRNA of a polypeptide having (i) an amino acid sequence as shown in SEQ ID No:
- 1 or (ii) an amino acid sequence at least 65% identical thereto,
- d) an antibody against a polypeptide having (i) an amino acid sequence as shown in SEQ ID No:
- 1 or (ii) an amino acid sequence at least 65% identical thereto, and
- e) a specific aptamer for a polypeptide having (i) an amino acid sequence as shown in SEQ ID No:
- 1 or (ii) an amino acid sequence at least 65% identical thereto.
A method of treating a cancer or an autoimmune disease comprising administering in a therapeutically effective amount an antagonist for a polypeptide having (i) an amino acid sequence as shown in SEQ ID No:
- 1 or (ii) an amino acid sequence at least 65% identical thereto, wherein the antagonist is selected from the group consisting of:
- a) a specific siRNA for a polypeptide having (i) an amino acid sequence as shown in SEQ ID No:
- 1 or (ii) an amino acid sequence at least 65% identical thereto,
- b) a gene antisense molecule for the gene encoding a polypeptide having (i) an amino acid sequence as shown in SEQ ID No:
- 1 or (ii) an amino acid sequence at least 65% identical thereto,
- c) a specific ribozyme for the mRNA of for a polypeptide having (i) an amino acid sequence as shown in SEQ ID No:
- 1 or (ii) an amino acid sequence at least 65% identical thereto,
- d) an antibody against a polypeptide having (i) an amino acid sequence as shown in SEQ ID No:
- I or (ii) an amino acid sequence at least 65% identical thereto, and
- e) a specific aptamer for a polypeptide having (i) an amino acid sequence as shown in SEQ ID No:
- 1 or (ii) an amino acid sequence at least 65% identical thereto.
A composition comprising an antagonist for a polypeptide having (i) an amino acid sequence as shown in SEQ ID No:
- 1 or (ii) an amino acid sequence at least 65% identical thereto, optionally together with a pharmaceutically acceptable carrier, for the treatment of a cancer or an autoimmune disease, wherein the antagonist is selected from the group consisting of:
- a) a specific siRNA for a polypeptide having (i) an amino acid sequence as shown in SEQ ID No:
- 1 or (ii) an amino acid sequence at least 65% identical thereto,
- b) an antisense molecule for the gene encoding a polypeptide having (i) an amino acid sequence as shown in SEQ ID No:
- I or (ii) an amino acid sequence at least 65% identical thereto,
- c) a specific ribozyme for the mRNA of a polypeptide having (i) an amino acid sequence as shown in SEQ ID No:
- 1 or (ii) an amino acid sequence at least 65% identical thereto,
- d) an antibody against a polypeptide having (i) an amino acid sequence as shown in SEQ ID No:
- 1 or (ii) an amino acid sequence at least 65% identical thereto, and
- e) a specific aptamer for a polypeptide having (i) an amino acid sequence as shown in SEQ ID No:
- 1 or (ii) an amino acid sequence at least 65% identical thereto.
A method for screening candidate compounds for at least one antagonist for a polypeptide having (i) an amino acid sequence as shown in SEQ ID No:
- 1 or (ii) an amino acid sequence at least 65% identical thereto with the ability to inhibit the propagation of a cell population, the method comprising the following steps:
- (i) contacting a cell population with a candidate compound, thereby enabling the introduction of the candidate compound into the cells of the cell population,
- (ii) cultivating the cell population for a time period sufficient to allow the candidate compound to be effective, and parallel cultivating a control cell population which has not been contacted with the candidate compound, and
- (iii) monitoring cell growth, cell properties, or a combination thereof in the cell population and in the control cell population,
- wherein a reduced growth, altered cell properties, or a combination thereof as compared to the control cell population is indicative that the candidate compound is an antagonist for the aforementioned polypeptide which inhibits the propagation of a cell population.
A method of treating a patient having a disease, which is caused by the propagation of an undesired cell population, the method comprising introducing an antagonist of a polypeptide having an (i) amino acid sequence as shown in SEQ ID No:
- 1 or (ii) an amino acid sequence at least 65% identical thereto into the patient in a therapeutically effective amount.
Dependencies are inferred from the claim text.