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US 2008/0166328 A1Published application
In vitro expansion of postpartum-derived cells using microcarriers
AssigneeEthicon, Inc.
InventorsAlexander M. Harmon, L.S. Klaudyne Hong, Anthony J. Kihm, Anna Gosiewska
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Abstract
Compositions and methods for the growth and expansion of mammalian cells in culture are provided. In particular, methods for the growth and expansion of postpartum-derived cells in vitro are provided using surfaces such as microcarrier beads.
Claims30
A method of culturing anchorage-dependent postpartum cells comprising,
- providing at least one anchorage-dependent postpartum cell;
- providing a cell growth medium for growing the postpartum cell;
- providing at least one carrier particle for attachment of the anchorage-dependent postpartum cell;
- and
- contacting the anchorage-dependent cell with the carrier particle in the presence of the growth medium under conditions permitting attachment and growth of the cell,
- thereby culturing the anchorage-dependent postpartum cell.
3 more elements
Dependencies are inferred from the claim text.