Method for Amplification of Endothelial Progenitor Cell in Vitro
AssigneesFoundation for Biomedical Research and Innovation, Tokai University Educational System
InventorsTakayuki Asahara, Haruchika Masuda
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Abstract
The present invention provides a method for expanding an endothelial progenitor cell in vitro. More particularly, the present invention provides a method for culturing a hemangioblast comprising incubating a hemangioblast in a serum-free culture medium containing one or more factors selected from the group consisting of stem cell growth factor, interleukin-6, FMS-like tyrosine kinase 3 and thrombopoietin, and a vascular endothelial cell produced by the method; and a serum-free culture medium containing one or more factors selected from the group consisting of stem cell growth factor, interleukin-6, FMS-like tyrosine kinase 3 and thrombopoietin, and a kit for the preparation of the serum-free culture medium and the like.
Claims16
A method for expanding a hemangioblast, which comprises incubating the hemangioblast in a serum-free culture medium containing a stem cell factor, interleukin-6, FMS-like tyrosine kinase 3 and thrombopoietin to proliferate an endothelial progenitor cell from the hemangioblast.
(canceled)
A serum-free culture medium containing a stem cell factor, interleukin-6, FMS-like tyrosine kinase 3 and thrombopoietin.
A kit for preparing a serum-free culture medium containing a stem cell factor, interleukin-6, FMS-like tyrosine kinase 3 and thrombopoietin, which comprises a stem cell factor, interleukin-6, FMS-like tyrosine kinase 3, thrombopoietin and a serum-free culture medium (at least one factor from a stem cell factor, interleukin-6, FMS-like tyrosine kinase 3 and thrombopoietin is not contained in the serum-free culture medium).
A method for culturing a hemangioblast, which comprises incubating the hemangioblast in a serum-free culture medium containing a stem cell factor, interleukin-6, FMS-like tyrosine kinase 3 and thrombopoietin.
Dependencies are inferred from the claim text.