Method of extensive culture of antigen-specific cytotoxic T cells
OwnerHiroaki Sagawa, Mitsuko Ideno +1inventors
InventorsHiroaki Sagawa, Mitsuko Ideno, Ikunoshin Kato
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Abstract
The present invention provides methods for inducing, maintaining and expanding CTL (cytotoxic T cell) having an antigen-specific cytotoxic activity at a high level, which is useful in the adoptive immunotherapy, by using as an effective ingredient at least one compound selected from the group consisting of acidic polysaccharides, acidic oligosaccharides, acidic monosaccharides, and salts thereof. The above-mentioned compounds include fucoidans, heparins, alginic acid, chondroitin sulfate A, chondroitin sulfate B, pectic acid, hyaluronic acid, degradation products of fucoidans, sulfated glucose, sulfated fucose and salts thereof.
Claims23
A method for inducing a cytotoxic T cell having an antigen-specific cytotoxic activity from a precursor cell capable of differentiating to a cytotoxic T cell, which comprises:
- incubating a precursor cell capable of differentiating to cytotoxic T cell with an antigen presenting cell in the presence of at least one compound selected from the group consisting of acidic polysaccharides, acidic oligosaccharides, acidic monosaccharides, and salts thereof.
A method for maintaining cytotoxic T cell having an antigen-specific cytotoxic activity, wherein the method comprises:
- (1) incubating a precursor cell capable of differentiating to cytotoxic T cell with an antigen presenting cell in the presence of at least one compound selected from the group consisting of acidic polysaccharides, acidic oligosaccharides, acidic monosaccharides and salts thereof, to induce a cytotoxic T cell having an antigen-specific cytotoxic activity, and
- (2) continuously culturing the induced cytotoxic T cell obtained from step (1) in the presence of at least one compound selected from the group consisting of acidic polysaccharides, acidic oligosaccharides, acidic monosaccharides, and salts thereof.
A method for expanding a cytotoxic T cell having an antigen-specific cytotoxic activity, which comprises:
- (1) incubating a precursor cell capable of differentiating to cytotoxic T cell with an antigen presenting cell in the presence of at least one compound selected from the group consisting of acidic polysaccharides, acidic oligosaccharides, acidic monosaccharides and salts thereof, to induce a cytotoxic T cell having an antigen-specific cytotoxic activity, and
- (2) incubating the induced cytotoxic T cell obtained by step (1) in the presence of at least one compound selected from the group consisting of acidic polysaccharides, acidic oligosaccharides, acidic monosaccharides and salts thereof.
A method for expanding a cytotoxic T cell having an antigen-specific cytotoxic activity, which comprises:
- incubating the cytotoxic T cell having an antigen-specific cytotoxic activity in the presence of at least one compound selected from the group consisting of:
- acidic polysaccharides, acidic oligosaccharides, acidic monosaccharides and salts thereof.
Dependencies are inferred from the claim text.