Masking Member, Light Measuring Method, Light Measuring Kit and Light Measuring Container

A masking member applied to a microplate having a plurality of wells in each of which is accommodated a liquid containing a measurement object, and being used for measurement of one of fluorescence and light emission, originated from the measurement object accommodated in each of the wells, through each bottom of the wells, said masking member comprising:

• a plurality of light shielding parts, being prepared corresponding to each of the wells, each having liquid permeability, and having light shielding effect for shielding a background light traveling from the liquid, which is located on the opposite side of the bottom of the corresponding well across the measurement object accommodated in the corresponding well, toward the bottom of the corresponding well;
• and
• a supporting structure including a sheet-shaped part covering an upper plane of the microplate, and a plurality of outer frame parts each being prepared corresponding to one of the wells for positioning the corresponding one of said light shielding parts on the opposite side of the bottom of the corresponding well across the measurement object accommodated in the corresponding well, while supporting the corresponding one of said light shielding parts.

A light measuring container accommodating a measurement object together with a liquid therein and measuring one of fluorescence and light emission, originated from the measurement object, through the bottom thereof,

• wherein a positioning means is provided on an inner wall of said container, for positioning a masking member with liquid permeability and also light shielding effect for shielding a background light traveling from a liquid, which is located on the opposite side of the bottom of said container across the measurement object, toward the bottom of said container.

A light measuring container comprising a plurality of wells each accommodating a liquid containing a measurement object, and being used for measuring one of fluorescence and light emission, originated from a measurement object accommodated in each of the wells, through each bottom of the wells respectively,

• wherein a positioning means is provided on an inner wall of each of the wells, for positioning a masking member having liquid permeability and shielding a background light traveling from a liquid, which is located on the opposite side of the bottom of the corresponding well across the measurement object accommodated in the corresponding well, toward the bottom of the corresponding well.