US 2008/0164149 A1Published application

Rapid gel electrophoresis system

OwnerMatthew R. Artzinventor

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Abstract

A rapid electrophoresis system [1000] provides rapid separation of an unknown sample [1] into its component compounds. An unknown sample [1] which is to be identified is placed in an inert electrophoresis gel [1142] in a gel container [1140] in housing [1110]. The housing [1110] has electric plates [1151, 1153] straddling housing [1110]. A plurality of temperature sensors measure the temperature or chemical breakdown of gel [1142] in each track [1131, 1133, 1135, 1137] running the length of the gel container [1140]. The tracks [1131, 1133, 1135, 1137] are monitored, selectively cooled or heated by a thermal controller [1210] to bring them all to a pre-defined optimum temperature. The power across the plates is also adjusted by power controller [1210] to prevent heating and the gel [1142] from being chemically altered so that it maintains its functionality, while providing optimum performance. The temperature at each location and time, and the progression of the components could be standardized to compare current results to stored results of other components.

Claims18

Dependent claims

A rapid electrophoresis system [1000] for separating an unknown sample [1] in an electrophoresis gel [1142] comprising:

a. A power source [1190] having first pole and a second pole, for providing a power across its poles;
b. A housing [1110] filled with an ionic solution [1155] and a gel container [1140] holding said gel [1142] with said unknown sample [1] immersed within the ionic solution [1155], the housing [1110] having a front end, and a back end, at least one inlet [1250], and at least one outlet [1350];
c. a first electric plate [1151], and a second electric plate [1153], the first electric plate being positioned near the front end of housing [1110] with the second electric plate [1153] being positioned at the rear of the housing [1110], the first electric plate being coupled to the first pole of the power source [1190];
and second electric plate [1153] being coupled to the second pole of the power source.
d. at least one sensor [1170] for sensing physical properties of at least one portion of the gel [1142];
e. a reservoir [1270] coupled to the housing for receiving the ionic solution [1155];
f. a pump [1300] coupled to the reservoir, and the housing [1110], the pump receiving ionic solution [1155] from the reservoir [1270] and circulating the ionic solution [1155] into the housing [1110], past the gel container [1140], out of housing [1110] and into the reservoir [1270];
g. a thermal controller [1230] coupled to the sensor [1170], the reservoir [1270], for determining if the ionic solution [1155] should cooled and operating the reservoir [1270] to interactively adjusting the temperature of the ionic solution [1155] accordingly.

5 more elements

A rapid electrophoresis system [1000] for separating an unknown sample [1] in an electrophoresis gel [1142] comprising:

a. A power source [1190] having a first pole and a second pole, for providing a power across its poles;
b. A housing [1110] filled with an ionic solution [1155], a gel container [1140] having a plurality of tracks [1131, 1133, 1135, 1137] each for holding said gel [1142] with said unknown sample [1], and a plurality of conduits [1141, 1143, 1145, 1147] in thermal communication with the tracks [1131, 1133, 1135, 1137] immersed within the ionic solution [1155], the housing [1110] having a front end, and a back end, at plurality of inlets [1251, 1253, 1255, 1257] each having a activatable valve [1251, 1253, 1255, 1257] capable of restricting flow through their respective inlets, and at least one outlet [1350];
c. a first electric plate [1151], and a second electric plate [1153], the first electric plate being positioned near the front end of housing [1110] with the second electric plate [1153] being positioned at the rear of the housing [1110], the first electric plate being coupled to the first pole of the power source [1190];
and the second electric plate being coupled to the second pole of the power source [1190].
d. at least one sensor [1170] in each track [1131, 1133, 1135, 1137] of gel container [1140] for sensing physical properties of at least one portion of the gel [1142] in each track;
e. a reservoir [1270] coupled to the housing for receiving the ionic solution [1155], and for cooling the ionic solution [1155] provided to it;
f. a pump [1300] coupled to the reservoir [1270], and the housing [1110], the pump receiving ionic solution [1155] from the reservoir [1270] and circulating the ionic solution [1155] into the housing [1110], past the gel container [1140], out of housing [1110] and into the reservoir [1270];
g. a thermal controller [1230] coupled to the sensor [1170], the reservoir [1270], the valves [1261-1267] for determining if the ionic solution [1155] should be cooled and operating the reservoir [1270] to interactively adjusting the temperature of the ionic solution [1155] and the valves [1261-1267] to adjust the amount of fluid flow through conduits [1143-1147] accordingly.

5 more elements

A rapid electrophoresis system [1000] for separating an unknown sample [1] in an electrophoresis gel [1142] comprising:

a. a power source [1190] having a first pole and second pole, for providing a power across its poles;
b. a housing [1110] filled with an ionic solution [1155], a gel container [1140] having a plurality of tracks [1131, 1133, 1135, 1137] each for holding said gel [1142] with said unknown sample [1], and a plurality of refrigeration units [1051, 1053, 1055, 1057] in thermal communication with the tracks [1131, 1133, 1135, 1137] immersed within the ionic solution [1155], the housing [1110] having a front end, and a back end, at least one inlets [1250], and at least one outlet [1350];
c. a first electric plate [1151], and a second electric plate [1153], the first electric plate being positioned near the front end of housing [1110] with the second electric plate [1153] being positioned at the rear of the housing [1110], the first electric plate being coupled to the first pole of the power source [1190];
and the second electric plate being coupled to the second pole.
d. at least one sensor [1170] in each track [1131, 1133, 1135, 1137] of gel container [1140] for sensing physical properties of at least one portion of the gel [1142] in each track;
e. a reservoir [1270] coupled to the housing for receiving the ionic solution [1155], and for cooling the ionic solution [1155] provided to it;
f. a pump [1300] coupled to the reservoir, and the housing [1110], the pump receiving ionic solution [1155] from the reservoir [1270] and circulating the ionic solution [1155] into the housing [1110], past the gel container [1140], out of housing [1110] and into the reservoir [1270];
g. a thermal controller [1230] coupled to the sensor [1170], the reservoir [1270], the valves [1261-1267] for determining if the ionic solution [1155] should be cooled and operating the refrigeration units [1051-1057] accordingly.

5 more elements

Dependencies are inferred from the claim text.