Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources
AssigneePacific Biosciences of California, Inc.
InventorsPaul Lundquist, Denis Zaccarin, Yves Lacroix, Stephen Turner, John Dixon
Life of this patent
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Abstract
Methods and systems for real-time monitoring of optical signals from arrays of signal sources, and particularly optical signal sources that have spectrally different signal components. Systems include signal source arrays in optical communication with optical trains that direct excitation radiation to and emitted signals from such arrays and image the signals onto detector arrays, from which such signals may be subjected to additional processing.
Claims16
1-49. (canceled)
A method of analyzing a plurality of signal sources, comprising:
- providing a substrate having a plurality of discrete signal sources disposed thereon;
- illuminating a first plurality of illuminated signal sources and a second plurality of illuminated signal sources, wherein the first plurality of illuminated signal sources are spaced apart from the second plurality of illuminated signal sources by a distance that is greater than a cross sectional dimension of an image of the signal source imaged on a detector array;
- and
- imaging the signal source on a detector array.
A method of analyzing a plurality of signal sources, comprising:
- providing a substrate having a plurality of discrete signal sources disposed thereon and oriented in at least a first and second rows of signal sources, wherein the first and second rows of signal sources are spaced apart by a first distance;
- directing excitation light in at least first and second linear illumination profiles at the first and second rows of signal sources, respectively;
- wherein the first distance is greater than a width of the first linear illumination profiles;
- and
- analyzing signals from the plurality of discrete signal sources in response to the excitation light.
A method of detecting fluorescent signals from a plurality of signal sources on a substrate, comprising directing excitation radiation at portions of the substrate occupied the plurality of signal sources on a substrate while not directing excitation radiation at portions of the substrate not occupied by the signal sources.
A method of analyzing a plurality of signal sources on a substrate, comprising:
- providing at least first and second adjacent signal sources on a substrate;
- selectively directing excitation radiation at the first and second signal sources while not substantially illuminating space between the first and second signal sources.
Dependencies are inferred from the claim text.