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OwnerNianqiang Wu, William Phillip Petros +2inventors
InventorsNianqiang Wu, William Phillip Petros, Daniel Charles Flynn, Peter Mico Gannett
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The present invention details a nanostructured electrochemical biosensor based on aptamer as the molecular recognition probe. The biosensor is comprised of an electrochemical cell that can be plugged into an electric controller. This electrochemical biosensor contains a working electrode made of the micro-/nano-scale gold dot array pattern, on which aptamer is immobilized on the surface of the dot array as the molecular recognition probe. The aptamer is labeled with an electrochemical indicator. Reversible binding of an analyte to the aptamer causes the change in the conformation of aptamer, consequently brings the electrochemical indicator close to the electrode surface. This results in the electron transfer from the electrochemical indicator to the electrode, which can be read as a change in output current or potential. The invented biosensor is a portable point-of-care device that has higher sensitivity, allows the measurement of the analyte more rapidly and requires much less sample volume than is presently available with current methods of detection.
An electrochemical cell comprising a non-conductive substrate, a plurality of electrodes connected to said substrate wherein said electrodes are also connected to electrical contacts and at least one of said electrodes is a working electrode wherein said working electrode is a gold dot micro/-nano patterned array with a molecular recognition probe wherein said molecular recognition probe contains an electrochemical redox indicator resulting in said molecular recognition probe binding to an analyte and said molecular recognition probe undergoing a conformational change causing said electrochemical indicator to alter the distance from said gold dot array surface.
A method to reversibly bind cisplatin in a serum comprising exposing an electrochemical cell to a serum wherein said electrochemical cell contains a working electrode, a reference electrode and a counter electrode connected to a non-conductive substrate and electrical contacts, binding an the aptamer [Seq1] labeled with an electrochemical redox indicator wherein said aptamer is immobilized on a micro/nano patterned gold dot array working electrode surface of an electrochemical cell wherein cisplatin binds to said aptamer causing a conformational change in said aptamer causing a change in the current density to said electrode.
A method of making an electrochemical cell comprising preparing an aptamer, adding a redox active group to said aptamer, attaching said aptamer to a gold dot micro/-nano patterned arrayworking electrode.
Dependencies are inferred from the claim text.