Methods and systems for multidimensional concentration and separation of biomolecules using capillary isotachophoresis
AssigneeCalibrant Biosystems, Inc.
InventorBrian M. Balgley
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Abstract
The invention provides a method for performing off-line multi-dimensional separation and analysis of a heterogeneous biomolecular sample. The method includes separating the heterogeneous biomolecular sample into a plurality of fractions using an, at least partially, capillary isotachophoresis mechanism. The plurality of fractions are then transferred to a liquid chromatography apparatus where they are each separated into a plurality of sub-fractions. The sub-fractions are then analyzed to determine their constituent molecules.
Claims23
A method for performing multi-dimensional separation and analysis of a heterogeneous biomolecular sample, the method comprising:
- introducing the heterogeneous biomolecular sample into an electrophoresis capillary;
- separating the heterogeneous biomolecular sample into a plurality of fractions using at least capillary isotachophoresis;
- introducing at least one of the plurality of fractions into at least one liquid chromatography capillary;
- concentrating the at least one of the plurality of fractions at a first end of the at least one liquid chromatography capillary;
- separating the at least one of the plurality of fractions into a plurality of sub-fractions using the at least one liquid chromatography capillary;
- and
- eluting each of the plurality of sub-fractions from a second end of the at least one liquid chromatography capillary.
A system for performing multi-dimensional separation and analysis of a heterogeneous biomolecular sample, comprising:
- an electrophoresis apparatus that separates the heterogeneous biomolecular sample into a plurality of fractions by at least capillary isotachophoresis;
- a liquid chromatography apparatus that concentrates at least one of the plurality of fractions and separates the at least one of the plurality of fractions into a plurality of sub-fractions;
- and
- a transfer mechanism that transfers at least one of the plurality of fractions from the electrophoresis apparatus into the liquid chromatography apparatus.
Dependencies are inferred from the claim text.