US 2008/0138894 A1 · PURIFIED HEPATITIS C VIRUS ENVELOPE PROTEINS FOR DIAGNOSTIC AND THERAPEUTIC USE

Abstract

The present invention relates to a method for purifying recombinant HCV single or specific oligomeric envelope proteins selected from the group consisting of E1 and/or E1/E2 characterized in that upon lysing the transformed host cells to isolate the recombinantly expressed protein a disulphide bond cleavage or reduction step is carried out with a disulphide bond cleavage agent. The present invention also relates to a composition isolated by such a method. The present invention also relates to the diagnostic and therapeutic application of these compositions. Furthermore, the invention relates to the use of HCV E1 protein and peptides for prognosing and monitoring the clinical effectiveness and/or clinical outcome of HCV treatment.

Claims13

Dependent claims

A recombinant vector comprising a vector sequence and an:

appropriate eukaryotic or viral promoter sequence operably linked to a nucleotide sequence comprising an HCV signal sequence attached before a sequence encoding an HCV envelope E2 protein starting at amino acid position 384 and ending in the region between amino acid positions 600 and 820 and wherein said HCV envelope E2 protein is produced by recombinant expression in lower eukaryotic or mammalian host cells.

Dependencies are inferred from the claim text.

Facts

Application

11/678,513Filed 23 Feb 2007

Priority

EP 94870132.129 Jul 1994

Published

12 Jun 2008

Term ends

Not estimatedThe source records don’t support an estimate.

Classification22 codes

C07K 16/118
Togaviridae ; Matonaviridae ; Flaviviridae: hepatitis C virus; GB virus C [GBV-C]
A61K 39/12
Medicinal preparations containing antigens or antibodies: viral antigens
A61K 39/29
Viral antigens: hepatitis virus
A61P 1/16
Drugs for disorders of the alimentary tract or the digestive system: for liver or gallbladder disorders, e.g. hepatoprotective agents,…

Family

Application08/612,973Division · filed 11 Mar 1996

Application08/928,017Division · filed 11 Sep 1997

Application09/899,303Division · filed 6 Jul 2001

Application11/678,513Division · filed 23 Feb 2007

US 2003/0036110 A1Published application

US 6,150,134 ARelated patent

US 2008/0138894 A1This patent

From the US filing history. Foreign family members aren’t listed.

Purified hepatitis c virus envelope proteins for diagnostic and therapeutic use

Life of this patent

Purified hepatitis c virus envelope proteins for diagnostic and therapeutic use

Life of this patent

Abstract

Claims13