Method and apparatus for using light to enhance cell growth and survival
AssigneeCardiac Pacemakers, Inc.
InventorsDarrell Wagner, Haris Sih, Steven Girouard
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Abstract
A biologic agent preparation system is provided for producing one or more biologic agents in vitro including pro-growth factors and/or pro-survival factors from cells. The one or more biologic agents are produced in response to exposure of mammalian cells to light. A biologic agent delivery system is also provided that delivers the pro-growth factors and/or pro-survival factors to an injured tissue region, or delivers cells treated with the pro-growth factors and/or pro-survival factors to the injured tissue region.
Claims35
A method for preparing a composition comprising one or more pro-growth or pro-survival factors, comprising:
- providing one or more light sources and a first population of mammalian cells in media;
- exposing the first population of mammalian cells to an amount of a wavelength of light or a band of wavelengths of light for a time so as to increase the amount of one or more pro-growth factors and/or one or more pro-survival factors in the media, yielding a composition comprising the one or more pro-growth factors and/or one or more pro-survival factors;
- and
- isolating the composition.
A method to identify a wavelength of light that enhances expression of pro-growth factors and/or pro-survival factors comprising:
- a) contacting in vitro a first population of mammalian cells in culture media with a wavelength of light or a band of wavelengths of light;
- b) collecting the media and contacting the media with a second population of mammalian cells which is different than the first population of cells;
- and
- c) identifying whether the media enhances survival or growth of the second population of cells.
Dependencies are inferred from the claim text.