Dicer substrate rna peptide conjugates and methods for rna therapeutics

A dicer substrate peptide conjugate comprising a double stranded ribonucleic acid (dsRNA) having a sense strand and an antisense strand and a double-stranded region of from 25 to 30 base pairs, and a peptide comprising SEQ ID NO:

• 34 or a variant thereof, wherein the dsRNA is conjugated to the peptide.

A dicer substrate peptide conjugate comprising a double stranded ribonucleic acid (dsRNA) having a sense strand and an antisense strand and a double-stranded region of from 25 to 30 base pairs, and a peptide comprising SEQ ID NO:

• 35 or a variant thereof, wherein the dsRNA is conjugated to the peptide.

A dicer substrate peptide conjugate comprising a double stranded ribonucleic acid (dsRNA) having a sense strand and an antisense strand and a double-stranded region of from 25 to 30 base pairs, and a peptide comprising SEQ ID NO:

• 38 or a variant thereof, wherein the dsRNA is conjugated to the peptide.

A dicer substrate peptide conjugate comprising a double stranded ribonucleic acid (dsRNA) having a sense strand and an antisense strand and a double-stranded region of from 25 to 30 base pairs, and a peptide comprising a PreS2 translocation motif or a variant thereof, wherein the dsRNA is conjugated to the peptide.

A dicer substrate peptide conjugate comprising a double stranded ribonucleic acid (dsRNA) having a sense strand and an antisense strand and a double-stranded region of from 25 to 30 base pairs, and a peptide comprising SEQ ID NO:

• 45 or a variant thereof, wherein the dsRNA is conjugated to the peptide.

A dicer substrate peptide conjugate comprising a double stranded ribonucleic acid (dsRNA) having a sense strand and an antisense strand and a double-stranded region of from 25 to 30 base pairs, and a chimeric peptide comprising penatratin or a variant thereof, and a polyarginine comprising at least five arginine residues, wherein the dsRNA is conjugated to the chimeric peptide.

A dicer substrate peptide conjugate comprising a double stranded ribonucleic acid (dsRNA) having a sense strand and an antisense strand and a double-stranded region of from 25 to 30 base pairs, and a peptide comprising SEQ ID NO:

• 58 or a variant thereof, wherein the dsRNA is conjugated to the peptide.

A dicer substrate peptide conjugate comprising a double stranded ribonucleic acid (dsRNA) having a sense strand and an antisense strand and a double-stranded region of from 25 to 30 base pairs, and a peptide comprising SEQ ID NO:

• 59 or a variant thereof, wherein the dsRNA is conjugated to the peptide.

A dicer substrate peptide conjugate comprising a double stranded ribonucleic acid (dsRNA) having a sense strand and an antisense strand and a double-stranded region of from 25 to 30 base pairs, and a peptide comprising SEQ ID NO:

• 60 or a variant thereof, wherein the dsRNA is conjugated to the peptide.

A dicer substrate peptide conjugate comprising a double stranded ribonucleic acid (dsRNA) having a sense strand and an antisense strand and a double-stranded region of from 25 to 30 base pairs, and a peptide comprising SEQ ID NO:

• 61 or a variant thereof, wherein the dsRNA is conjugated to the peptide.

A dicer substrate peptide conjugate comprising a double stranded ribonucleic acid (dsRNA) having a sense strand and an antisense strand and a double-stranded region of from 25 to 30 base pairs, and a peptide comprising SEQ ID NO:

• 62 or a variant thereof, wherein the dsRNA is conjugated to the peptide.

A dicer substrate peptide conjugate comprising a double stranded ribonucleic acid (dsRNA) having a sense strand and an antisense strand and a double-stranded region of from 25 to 30 base pairs, and a peptide comprising from about 5 to about 100 amino acids, wherein the peptide comprises at least two positively charge amino acid residues, and wherein the dsRNA is conjugated to the peptide.