Method for chemical transformation using a mutated enzyme
AssigneeBioCatalytics, Inc.
InventorJ. Rozzell
Life of this patent
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Abstract
Methods for chemically transforming compounds using a mutated enzyme are provided, and more particularly a method for the production of an amino acid from a target 2-ketoacid, the production of an amine from a target ketone and the production of an alcohol from a target ketone. The methods comprise creating a mutated enzyme that catalyzes the reductive amination or transamination of the target 2-ketoacid or ketone or the reduction of the ketone and providing the mutated enzyme in a reaction mixture comprising the target 2-ketoacid or ketone under conditions sufficient to permit the formation of the desired amino acid, amine or alcohol to thereby produce the amino acid, amine or alcohol.
Claims30
A method for the production of an amino acid from a target 2-ketoacid comprising:
- creating a mutated enzyme that catalyzes the reductive amination or transamination of the target 2-ketoacid;
- and
- providing the mutated enzyme in a reaction mixture comprising the target 2-ketoacid under conditions sufficient to permit the formation of the amino acid to thereby produce the amino acid.
A method for the production of an amine from a target ketone comprising:
- creating a mutated enzyme that catalyzes the reductive amination or transamination of the target ketone;
- and
- providing the mutated enzyme in a reaction mixture comprising the target ketone under conditions sufficient to permit the formation of the amine to thereby produce the amine.
A method for the production of an alcohol from a target ketone comprising:
- creating a mutated enzyme that catalyzes the reduction of the target ketone;
- and
- providing the mutated enzyme in a reaction mixture comprising the target ketone under conditions sufficient to permit the formation of the alcohol to thereby produce the alcohol.
Dependencies are inferred from the claim text.