Methods and Compositions for Degradation of Lignocellulosic Material
AssigneeDyadic International, Inc.
InventorsMarco Baez-Vasquez, Richard Burlingame, Jon Magnuson, Marion Bradford, Arkady Sinitsyn
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Abstract
Methods to convert lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material are provided, as well as novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass.
Claims123
A multi-enzyme product comprising a product of a Chrysosporium including Chrysosporium lucknowense and exhibiting:
- a glucoamylase activity of at least about 0.05 U per milligram of dry matter, a β-glucosidase activity of at least about 22 nmol/min/mg of dry matter, and an α-arabinofuranosidase activity of at least about 9 nmol/min/mg of dry matter.
A multi-enzyme product comprising a product of a Trichoderma including Trichoderma reesei and exhibiting:
- a glucoamylase activity of at least about 0.001 U per milligram of dry matter, a β-xylosidase activity of at least about 280 nmol/min/mg of dry matter, a β-glucosidase activity of at least about 454 nmol/min/mg of dry matter, and an α-arabinofuranosidase activity of at least about 100 nmol/min/mg of dry matter.
A multi-enzyme product comprising a product of a Aspergillus including Aspergillus japonicus and exhibiting:
- a glucoamylase activity of at least about 0.65 U per milligram of dry matter, a β-xylosidase activity of at least about 553 nmol/min/mg of dry matter, a β-glucosidase activity of at least about 167 nmol/min/mg of dry matter, and an α-arabinofuranosidase activity of at least about 62 nmol/min/mg of dry matter.
A multi-enzyme product comprising a product of a Penicillium including Penicillium funiculosum (Talaromyces including Talaromyces flavus) and exhibiting:
- a glucoamylase activity of at least about 0.18 U per milligram of dry matter, a β-xylosidase activity of at least about 8 nmol/min/mg of dry matter, a β-glucosidase activity of at least about 691 nmol/min/mg of dry matter, and an α-arabinofuranosidase activity of at least about 29 nmol/min/mg of dry matter.
A multi-enzyme product comprising a product of a Penicillium including Penicillium canescens (P. solitum) and exhibiting:
- a glucoamylase activity of at least about 0.08 U per milligram of dry matter, a β-xylosidase activity of at least about 15 nmol/min/mg of dry matter, a β-glucosidase activity of at least about 40 nmol/min/mg of dry matter, and an α-arabinofuranosidase activity of at least about 956 nmol/min/mg of dry matter.
A method for degrading a lignocellulosic material to fermentable sugars, comprising contacting the lignocellulosic material with an effective amount of a multi-enzyme product derived from one microorganism, whereby at least one fermentable sugar is produced, and wherein at least one of enzyme in the multi-enzyme product is derived from Trichoderma, Chrysosporium, Aspergillus, Penicillium (Talaromyces), or mutants of any of the foregoing.
A method for degrading a lignocellulosic material to fermentable sugars, comprising contacting the lignocellulosic material with an effective amount of a multi-enzyme product derived from one microorganism, whereby at least one fermentable sugar is produced, and wherein at least one of enzyme in the multi-enzyme product is derived from Penicillium funiculosum or a mutant derived therefrom.
A method for producing a multi-enzyme product capable of producing fermentable sugars, comprising cultivating a microorganism selected from the group consisting of Trichoderma, Chrysosporium, Aspergillus, Penicillium (Talaromyces) and mutants thereof in a nutrient medium, and recovering the multi-enzyme product from the fermentation broth.
A method for producing fermentable sugars, comprising cultivating a microorganism selected from the group consisting of Trichoderma, Chrysosporium, Aspergillus, Penicillium (Talaromyces) and mutants thereof in a nutrient medium comprising a lignocellulosic material, whereby fermentable sugars are produced.
Dependencies are inferred from the claim text.