Signal peptide for the production of recombinant proteins

An expression construct comprising:

• an expression signal;
• a DNA sequence coding for a signal peptide with a cleavage site to a recombinant protein, wherein the last three amino acids before the cleavage site are alanine-phenylalanine-alanine;
• and
• an in-frame linked recombinant gene coding for a recombinant protein.

A method for the fermentative production of a recombinant protein, the method comprising:

• a) culturing a microbe in a fermentation medium such that the microbe produces the recombinant protein in the form of a signal peptide-protein fusion product, the microbe selected from the group of microbes selected from the group consisting of:
• i) microbes having a DNA sequence coding for a signal peptide with a cleavage site to a recombinant protein, wherein the last three amino acids before the cleavage site are alanine-phenylalanine-alanine (AFA);
• ii) microbes having an expression construct B comprising an expression signal;
• a DNA sequence coding for a signal peptide with a cleavage site to a recombinant protein, wherein the last three amino acids before the cleavage site are alanine-phenylalanine-alanine;
• and an in-frame linked recombinant gene coding for a recombinant protein;
• and
• iii) microbes having a plasmid having the DNA sequence of i) or the expression construct of ii),
• wherein upon secretion of the signal peptide-protein fusion product through the cytoplasmic membrane into the periplasm, the signal peptide is cleaved off at a cleavage site between signal peptide and the recombinant protein with the recombinant protein being obtained in the periplasm or the fermentation medium;
• b) purifying the recombinant protein after the fermentation.