Method for producing recombinant proteins
AssigneeImmunex Corporation
InventorVictor Fung
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Abstract
A method for producing recombinant proteins, preferably fusion proteins comprising an Fc portion of an immunoglobulin molecule, more preferably fusion proteins comprising an extracellular domain of a tumor necrosis factor receptor fused to the Fc portion of an immunoglobulin molecule, is disclosed. The method of the present invention allows a reduction of misfolding of the protein, thereby giving rise to a higher yield of the desired protein.
Claims27
A method for production of recombinant protein comprising culturing a recombinant mammalian host cell which encodes and expresses said recombinant protein so as to produce said recombinant protein, and obtaining the recombinant protein so produced, wherein during a production phase in serum-free medium, said host cell is cultured at a temperature of 25-34° C., wherein said method results in reduction in disulfide scrambling in the recombinant protein produced compared to when carrying out the production phase at a temperature of 37° C., wherein said recombinant protein is a fusion protein of a first protein selected from the group consisting of FLT3L, CD40L, TRAIL and IL-1R type II, and a second protein comprising an Fc portion of an immunoglobulin molecule.
Dependencies are inferred from the claim text.