Maltose-1-phosphate-producing enzyme
AssigneeKao Corporation
InventorsKazuaki IGARASHI, Shuichi Takizawa, Norihiko Higaki, Jun Hitomi
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Abstract
The present invention provides an M-1-P-producing enzyme which employs, as starting materials, an oligosaccharide or polysaccharide having a glucose polymerization degree of 5 or more and containing an α-1,4-glycosidic bond such as maltooligosaccharide, dextrin, or starch, and which enables production of a large amount of M-1-P. The M-1-P-producing enzyme can produce M-1-P from a phosphoric acid or a salt thereof and an oligosaccharide or polysaccharide having a glucose polymerization degree of 5 or more and containing an α-1,4-glycosidic bond.
Claims23
A method for producing a maltose-1-phosphate-producing enzyme, comprising culturing a microorganism capable of producing a maltose-1-phosphate-producing enzyme which is capable of producing maltose-1-phosphate from a phosphoric acid or a salt thereof and an oligosaccharide or polysaccharide containing an α-1,4-glycosidic bond and having a glucose polymerization degree of 5 or more in a medium containing a phosphoric acid or a salt thereof in an amount of 50 mM or more, and collecting the maltose-1-phosphate-producing enzyme produced in the medium.
A medium for producing a maltose-1-phosphate-producing enzyme which is capable of producing a maltose-1-phosphate from a phosphoric acid or a salt thereof and an oligosaccharide or polysaccharide containing an α-1,4-glycosidic bond and having a glucose polymerization degree of 5 or more, comprising a phosphoric acid or a salt thereof in an amount of 50 mM or more.
A method for producing maltose-1-phosphate, comprising reacting a phosphoric acid or a salt thereof and an oligosaccharide or polysaccharide having a glucose polymerization degree of 5 or more and containing an α-1,4-glycosidic bond with a maltose-1-phosphate-producing enzyme which is capable of producing maltose-1-phosphate from a phosphoric acid or a salt thereof and an oligosaccharide or polysaccharide containing an α-1,4-glycosidic bond and having a glucose polymerization degree of 5 or more.
A method for producing maltose-1-phosphate, comprising culturing a microorganism capable of producing a maltose-1-phosphate-producing enzyme which is capable of producing maltose-1-phosphate from a phosphoric acid or a salt thereof and an oligosaccharide or polysaccharide containing an α-1,4-glycosidic bond and having a glucose polymerization degree of 5 or more in a medium containing 1 mM or more of a phosphoric acid or a salt thereof and an oligosaccharide or polysaccharide having a glucose polymerization degree of 5 or more and containing an α-1,4-glycosidic bond, and collecting maltose-1-phosphate produced and accumulated in the medium.
A gene encoding the protein selected from the group consisting of:
- (a) A protein having the amino acid sequence of SEQ ID NO:
- 2,
- (b) A protein having the amino acid sequence of SEQ ID NO:
- 2 in which one to several amino acid residues are deleted, substituted, or added, and which is capable of producing maltose-1-phosphate,
- (c) A protein which has an amino acid sequence exhibiting 60% or more homology with that of SEQ ID NO:
- 2 and which is capable of producing a maltose-1-phosphate, and
- (d) A protein which has an amino acid sequence exhibiting 38% or more homology with that of SEQ ID NO:
- 2, which is capable of producing a maltose-1-phosphate, and which contains an amino acid sequence represented by Ala-Glu-Asn-Pro-Pro-Lys-Lys(or Arg)-Tyr-Gln(or Glu)-Asp-Ile (SEQ ID NO:
- 11) or Phe-Arg-Val(or Ile)-Asp-Asn-Pro-His-Thr-Lys-Pro (SEQ ID NO:
- 12).
A maltose-1-phosphate-producing enzyme gene selected from the group consisting of:
- (a) A DNA which has a nucleotide sequence represented by SEQ ID NO:
- 1
- (b) A DNA which is capable of hybridizing a DNA fragment having the nucleotide sequence of (a) under stringent conditions and which encodes a protein capable of producing maltose-1-phosphate;
- and
- (c) A DNA which exhibits 60% or more homology with the nucleotide sequence of (a) and which encodes a protein capable of producing maltose-1-phosphate.
Dependencies are inferred from the claim text.