Modified cellulases with increased thermostability, thermophilicity, and alkalophilicity
AssigneeIogen Energy Corporation
InventorsPatrick St-Pierre, Nabil Masri, Marie-Christine Fournier, Theresa White
Life of this patent
Estimating the term…
Abstract
A modified Family 6 cellulase enzyme comprising a proline residue at position 413 is provided. Genetic constructs and genetically modified microbes comprising DNA sequences encoding the modified Family 6 cellulase are also provided. Family 6 cellulases of the invention display improved thermostability, thermophilicity, alkalophilicity, or a combination thereof, relative to the parent Family 6 cellulases. Such cellulases find use in a variety of applications in industry that require cellulase stability and activities at temperatures, pH values, or both, above that of the native enzyme.
Claims34
A modified Family 6 cellulase comprising a proline residue at position 413, said position determined from sequence alignment of said modified cellulase with a Trichoderma reesei Cel6A amino acid sequence as defined in SEQ ID NO:1, wherein said modified cellulase exhibits enhanced thermostability, alkalophilicity, thermophilicity or a combination thereof relative to a corresponding parent Family 6 cellulase.
A modified Family 6 cellulase selected from the group consisting of:
- (SEQ ID NO:
- 12)
- TrCe16A-S413P;
- (SEQ ID NO:
- 13)
- TrCe16A-G82E-G231S-N305S-R410Q-S413P;
- (SEQ ID NO:
- 14)
- TrCe16A-G231S-S413P;
- (SEQ ID NO:
- 15)
- TrCe16A-N305S-S413P;
- (SEQ ID NO:
- 16)
- TrCe16A-R410Q-S413P;
- (SEQ ID NO:
- 17)
- TrCe16A-G231S-N305S-S413P;
- (SEQ ID NO:
- 18)
- TrCe16A-G231S-R410Q-S413P;
- (SEQ ID NO:
- 19)
- TrCe16A-N305S-R410Q-S413P;
- (SEQ ID NO:
- 20)
- TrCe16A-G231S-N305S-R410Q-S413P;
- (SEQ ID NO:
- 21)
- HiCe16A-Y420P;
- and
- (SEQ ID NO:
- 22)
- PcCe16A-S407P.
Dependencies are inferred from the claim text.