Identifying haematopoietic stem cells based on cell surface markers
AssigneePeter MacCallum Cancer Institute
InventorsSusan Nilsson, Paul Simmons, David Haylock
Life of this patent
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Abstract
The present invention relates to a method of identifying a haematopoietic stem cell (HSC) or progeny thereof comprising the steps of: obtaining a cell sample including HSC or progeny thereof; detecting the presence of at least one carbohydrate sequence having a sequence of at least one disaccharide repeat of glucuronic acid and N-acetylglucosamine or an equivalent thereof; and identifying a HSC or progeny thereof having the sequence or equivalent thereof. The invention also relates to methods of enriching cell populations for HSC or progeny thereof, for isolating HSC or progeny thereof and cell preparations obtained using the methods of their invention and their uses.
Claims5
-46. (canceled)
A method of identifying a subpopulation of Lin−HA+haematopoietic stem cells (HSC) or progeny, thereof comprising the steps of:
- obtaining a cell sample including Lin HA+ HSC or progeny thereof;
- combining the sample with a marker which detects at least one disaccharide repeat unit of glucuronic acid and N-acetylglucosamine;
- detecting the presence of hyaluronic acid (HA) on a cell of the sample;
- and
- identifying as a HSC or progeny thereof a cell having HA on the cell.
Dependencies are inferred from the claim text.