Method for quantitation of collagen in tissue
AssigneeKCI Licensing, Inc.
InventorsPatricia A. Villanueva, Amy K. McNulty, Herbert D. Beniker, Kristine Kieswetter
Life of this patent
Estimating the term…
Abstract
For successful wound healing to occur, the newly formed skin must generate tensile strength through collagen deposition. Measurement of collagen in the granulating wound bed may be predictive of successful wound healing. Existing methods for collagen measurement either require specialized equipment, or do not allow for discrimination of potential interfering molecules. Herein described is an accurate, specific and reliable method to extract and quantify collagen from tissue utilizing high pressure liquid chromatography techniques. The method is sensitive enough to measure the small amounts of collagen found in newly healing wounds.
Claims17
A method of assessing wound healing progress comprising:
- (a) obtaining a tissue sample from a wound site of a subject;
- (b) processing the tissue sample;
- (c) separating glycine, proline, and hydroxyproline in the processed tissue sample by high-pressure liquid chromatography to obtain analyte peaks areas;
- (d) determining concentrations of the glycine, proline, and hydroxyproline in the tissue;
- (e) correlating the concentrations of the glycine, proline, and hydroxyproline in the tissue with an amount of total collagen in the tissue;
- and
- (f) comparing the amount of total collagen in the tissue at the wound site with the amount of collagen in non-wounded tissue to assess wound healing progress.
Dependencies are inferred from the claim text.