Methods and kits for determining sperm cell number
AssigneeDyn-BioShaf (2006) Ltd.
InventorShafrira Shai
Life of this patent
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Abstract
A flow cytometry method and kit is provided for quantifying an absolute number of mature sperm cells in a semen sample, the method comprising (a) determining a total particle number (T); (b) determining a number of DNA comprising particles (D); (c) determining a number of cells which are not mature sperm cells (B) and; (d) determining a background signal (A); wherein steps (a) to (d) are not effected in a single aliquot of the semen sample, and whereas the number of mature sperm cells in the semen sample is equal to [(T×D)/100]−(A+B), thereby quantifying the absolute number of mature sperm cells in the semen sample.
Claims24
A flow cytometry method for quantifying an absolute number of mature sperm cells in a semen sample, the method comprising:
- (a) determining a total particle number (T);
- (b) determining a number of DNA comprising particles (D);
- (c) determining a number of cells which are not mature sperm cells (B);
- and
- (d) determining a background signal (A);
- wherein steps (a) to (d) are not effected in a single aliquot of the semen sample, and whereas the number of mature sperm cells in the semen sample is equal [(T×D)/100]−(A+B), thereby quantifying the absolute number of mature sperm cells in the semen sample.
A flow cytometry method for quantifying a relative number of mature sperm cells in a semen sample, the method comprising:
- (a) determining a number of DNA comprising particles (D);
- (b) determining a number of cells which are not mature sperm cells (B);
- and
- (c) determining a background signal (A);
- wherein steps (a)-(c) are not effected in a single aliquot of the semen sample, and whereas the number of mature sperm cells in the semen sample is equal to D−(A+B), thereby quantifying the relative number of mature sperm cells in the semen sample.
A kit for determining a number of sperm cells in a semen sample, wherein the kit comprises:
- (i) a white blood cell specific antibody;
- (ii) a DNA staining agent;
- (iii) a plurality of fluorescent beads;
- and
- (iv) a plurality of tubes wherein each four of said tubes are packaged in a single packaging.
Dependencies are inferred from the claim text.