Recombinant P. falciparum merozoite protein-1 42 vaccine
AssigneeUnited States Army Medical Research and Command
InventorsEvelina Angov, Jeffrey Lyon, Christian Darko, Joe Cohen
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Abstract
In this application is described the expression and purification of a recombinant Plasmodium falciparum (FVO) MSP-142. The method of the present invention produces a highly purified protein that retains folding and disulfide bridging of the native molecule. The recombinant MSP-142 is useful as a diagnostic reagent, for use in antibody production, and as a vaccine.
Claims47
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A recombinant vector comprising a DNA sequence encoding MSP-142.
A method for producing and purifying recombinant P. falciparum FVO MSP-142 protein comprising:
- growing a host cell containing a vector expressing P. falciparum FVO MSP-142 proteins in a suitable culture medium,
- causing expression of said vector under suitable conditions for production of soluble MSP-142 protein and,
- lysing said host cells and recovering said MSP-142 protein such that it retains its native folding.
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Dependencies are inferred from the claim text.