Method for increasing tumor cell immunogenicity using heat shock protein
OwnerAnnie-Chen Traninventor
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Abstract
A method for induction of endogenous heat shock protein in tumor cells using a simple heat shock treatment which provides a simple and inexpensive method for augmenting antitumor vaccine potency. The method comprises administering to a mammal tumor cells which have been subject to a heat shock condition sufficient to cause induction of endogenous heat shock protein therein. Also disclosed is a composition for enhancing tumor cell immunogenicity comprising a therapeutically effective amount of attenuated tumor cells which have been subject to a heat shock condition.
Claims20
1-26. (canceled)
A method of enhancing tumor cell immunogenicity in a subject, comprising the steps of:
- (a) genetically modifying tumor cells for expression of GM-CSF;
- (b) irradiating said genetically modified tumor cells;
- (c) subjecting said genetically modified tumor cells to a heat shock condition selected from the group consisting of application of about forty three to forty four degrees centigrade for a duration of about thirty minutes to two hours;
- and
- (d) administering to said subject a therapeutically effective amount of said tumor cells which have been subjected to said heat shock condition,
- wherein the immunogenicity of said genetically modified and irradiated tumor cells which have been subjected to said heat shock condition is greater than that of genetically modified and irradiated tumor cells which have not been subjected to said heat shock condition.
A method for enhancing tumor cell immunogenicity comprising:
- (a) removing a tumor cell from a mammal;
- (b) subjecting said tumor cell to a heat shock condition selected from the group consisting of application of about forty three to forty four degrees centigrade for a duration of about thirty minutes to two hours;
- (c) irradiating said tumor cell to render it proliferation incompetent;
- and
- (d) administering said proliferation incompetent tumor cell to said mammal, wherein said tumor cell immunogenicity is greater than that of an irradiated tumor cell which has not been subjected to said heat shock condition.
A method of inhibiting growth of a tumor, relieving or regressing a tumor in a subject, comprising:
- (a) genetically modifying tumor cells to expression of GM-CSF;
- (b) irradiating said genetically modified tumor cells to generate attenuated tumor cells;
- (c) subjecting said genetically modified attenuated tumor cells to a heat shock condition selected from the group consisting of application of about forty three to forty four degrees centigrade for a duration of about thirty minutes to two hours;
- and
- (d) administering to said subject a therapeutically effective amount of said attenuated tumor cells which have been subjected to said heat shock condition,
- wherein growth of said tumor is inhibited to an extent greater than results from administering attenuated tumor cells which have not been subjected to said heat shock condition.
A method of enhancing expression of heat shock protein in a tumor cell, comprising the steps of:
- (a) genetically modifying tumor cells to express GM-CSF;
- (b) irradiating said genetically modified tumor cells;
- (c) subjecting said genetically modified tumor cells to a heat shock condition selected from the group consisting of application of about forty three to forty four degrees centigrade for a duration of about thirty minutes to two hours;
- and
- wherein the expression level of heat shock protein in said genetically modified and irradiated tumor cells subjected to said heat shock condition is greater than that of said genetically modified and irradiated tumor cells which have not been subjected to said heat shock condition.
Dependencies are inferred from the claim text.