Method and composition for treating diabetes
OwnerDavid Wolf, Donnie Ruddinventors
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Abstract
The present invention is directed to the expansion, preferably TVEMF-expansion, of mammalian blood stem cells in a rotating bioreactor, preferably a TVEMF-bioreactor, to compositions resulting from the expanded and TVEMF-expanded cells, and to a method of treating disease or repairing tissue with the compositions.
Claims37
A method of treating a diabetic condition comprising the step of administering to a mammal a therapeutically effective amount of a pharmaceutical blood stem cell composition comprising expanded blood stem cells wherein the blood stem cells have been expanded in a rotating bioreactor rotated about a substantially horizontal longitudinal axis to suspend the cells in a three-dimensional environment of the rotating bioreactor, wherein the expanded blood stem cells have a unique phenotypic expression as a result of the expansion, and wherein the expanded blood stem cells are not substantially differentiated.
A method of treating a diabetic condition comprising the step of administering to a mammal a therapeutically effective amount of a pharmaceutical blood stem cell composition comprising TVEMF-expanded blood stem cells wherein the blood stem cells have been expanded in a rotating TVEMF-bioreactor rotated about a substantially horizontal longitudinal axis to suspend the cells in a three-dimensional environment of the rotating TVEMF-bioreactor, wherein the expanded blood stem cells have a unique phenotypic expression as a result of the expansion, and wherein the expanded blood stem cells are not substantially differentiated.
A method of treating a diabetic condition of a mammal comprising the steps of:
- placing a blood mixture comprising adult stem cells in a culture chamber of a rotatable bioreactor;
- expanding the adult stem cells in the rotatable bioreactor by rotating the culture chamber of the rotatable bioreactor about its horizontal longitudinal central axis;
- removing the expanded adult stem cells from the rotatable bioreactor;
- preparing an expanded adult stem cell composition comprising the expanded adult stem cells;
- and
- administering to a mammal a therapeutically effective amount of the expanded adult cell composition to treat the diabetic condition of the mammal.
Dependencies are inferred from the claim text.