Methods and systems for determining a baseline during image processing
AssigneeFluidigm Corporation
InventorsSimant Dube, Gang Sun, Lian-She Zhao
Life of this patent
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Abstract
A method of determining a plurality of positions associated with a plurality of reaction chambers of a microfluidic device includes a) providing a baseline image; b) providing a template image of a reaction chamber; and c) selecting a region of the baseline image. The method also includes d) performing a matching process including matching the template image to one or more portions of the region of the baseline image; e) determining a position of a first chamber; and f) predicting a position of a second chamber. The method further includes g) repeating steps c) through f) for subsequent chambers.
Claims25
A method of determining a plurality of positions associated with a plurality of reaction chambers of a microfluidic device, the method comprising:
- a) providing a baseline image;
- b) providing a template image of a reaction chamber;
- c) selecting a region of the baseline image;
- d) performing a matching process including matching the template image to one or more portions of the region of the baseline image;
- e) determining a position of a first chamber;
- f) predicting a position of a second chamber;
- and
- g) repeating steps c) through f) for subsequent chambers.
A method of determining positions of a plurality of chambers of a microfluidic device, the method comprising:
- providing a passive reference image of the microfluidic device;
- determining a plurality of first chamber positions based on the passive reference image;
- providing an additional passive reference image of the microfluidic device;
- determining a plurality of second chamber positions based on the additional passive reference image;
- providing a reporter dye image of the microfluidic device;
- determining a plurality of third chamber positions based on the reporter dye image;
- and
- determining one or more statistical parameters based on the reporter dye image.
A method of removing a baseline from PCR ratio data, the method comprising:
- obtaining a plurality of images, each of the plurality of images associated with a cycle of a PCR process;
- determining a ratio of reporter dye to passive reference dye for each of the plurality of images, thereby providing a ratio data curve;
- determining a corner point of the ratio data curve;
- determining a baseline value associated with the ratio data curve;
- and
- subtracting the baseline value from each value in the ratio data curve, thereby providing a baseline removed ratio data curve.
A method of determining a change in threshold value for a plurality of ratio data curves, the method comprising:
- determining a first quality factor for a first ratio data curve of the plurality of ratio data curves;
- determining that the first quality factor is greater than a predetermined value;
- determining a second quality factor for a second ratio data curve of the plurality of ratio data curves;
- determining that the second quality factor is greater than the predetermined value;
- and
- determining the change in threshold value based on the first ratio data curve and the second ratio data curve.
Dependencies are inferred from the claim text.