Method and Kit for Determining the Immunisation Status of a Person

Serological method for determining the vaccine status of a person by detection and quantification of IgG-type serum antibodies specific to the vaccine antigens of a plurality of pathogenic agents of bacterial, viral, fungal or parasitic type, characterized in that a complex of immunological reactions is detected and quantified between each said vaccine antigen and respectively each said IgG-type antibody specific to said vaccine antigen, possibly present in a sample of human serum to be tested, by conducting the steps of:

• 1—Contacting one same said serum sample to be tested with:
• one same solid substrate on which a plurality of said vaccine antigens are fixed corresponding to a plurality of different pathogenic agents, preferably at least 3, further preferably at least 4 said vaccine antigens of different pathogenic agents, at different areas of the substrate,
• in the presence of at least one detection substance reacting by complexing with said IgG-type specific antibodies and not reacting with said vaccine antigens, and
• 2—The concentration of said IgG-type specific antibodies is determined by quantifying the complexes resulting from the reaction of at least one said detection substance with said IgG-type specific antibodies complexed with said vaccine antigens fixed onto said solid substrate.