Method of Lysis of Erythrocytes
AssigneeImmunotech
InventorsAndre van Agthoven, Jean-Pierre Daziano, John Maples
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Abstract
A multifunctional reagent for erythrocytes containing an amount sufficient to produce the lysis of erythrocytes or the sphering of erythrocytes in such a way that they can be detected by a cytometer or an automatic counting device, of a carbamate or of an agent inducing the formation by the erythrocytes, from carbonate and from a nitrogenated heterocycle or ammonium ions, of a carbamate combined with the absorption of CO2 by the erythrocytes, process for lysing or sphering erythrocytes and preparation process for leucocytes.
Claims25
A method of lysis of erythrocytes comprising:
- (a) providing a lysis reagent comprising a carbonic anhydrase and an ammonium salt or a heterocyclic amine or salt thereof;
- and
- (b) mixing a blood sample with said lysis reagent to lyse erythrocytes in formed mixture.
A method of lysis of erythrocytes comprising:
- (a) providing a lysis reagent comprising an ammonium salt or a heterocyclic amine or salt thereof and a catalyst agent enabling inducing formation of carbamate in the presence of erythrocytes;
- and
- (b) mixing a blood sample with said lysis reagent and forming a mixture, wherein said catalyst agent induces formation of carbamate in said mixture, thereby causing lysis of erythrocytes in said mixture.
A method of sphering erythrocytes comprising:
- (a) mixing a blood sample with an isotonic diluent and a lysis reagent comprising a carbonic anhydrase and an ammonium salt or a heterocyclic amine or salt thereof to sphere erythrocytes;
- and
- (b) analyzing sphered erythrocytes on a flow cytometer.
Dependencies are inferred from the claim text.