Methods of increasing protein expression levels
OwnerWilliam Brondyk, Xuliang Jiang +1inventors
InventorsWilliam Brondyk, Xuliang Jiang, Rene Schweickhardt
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Abstract
The present invention relates to methods of increasing protein expression levels whereby at least one amino acid in a protein amino acid sequence is substituted for the amino acid, proline. Preferably, the substitution occurs within 15 amino acids, more preferably within 10 amino acids and most preferably within 5 amino acids of a cysteine amino acid residue. The present invention not only includes methods for polypeptides with proline substitutions, but also polynucleotides with codon substitutions for which a codon for any amino acid, except proline, is substituted for a codon encoding for proline.
Claims7
An isolated nucleic acid comprising a sequence that encodes a mutant of a native polypeptide wherein the native polypeptide consists of the amino acid sequence of SEQ ID NO:1, and wherein the amino acid at position 23 or the amino acid at position 76 of SEQ ID NO:1 is substituted with proline and wherein said mutant exhibits increased expression compared with that of the native polypeptide.
Dependencies are inferred from the claim text.