Method and Kit for the Early Detection of Impaired Renal Status

A method of performing an assay on a body fluid of a mammalian subject receiving intra-operative administration of aprotinin during cardio-pulmonary bypass surgery and identifying the presence of urinary aprotinin therein, the method comprising the steps of:

• a. providing a kit for point-of-care measurement of urinary aprotinin;
• b. providing a urine sample obtained from a mammalian subject receiving intra-operative administration of aprotinin during cardio-pulmonary bypass surgery;
• c. placing the sample in the kit;
• and
• d. identifying the presence of urinary aprotinin in the sample, wherein the presence or absence of urinary aprotinin directs a caregiver's therapeutic decision regarding the intra-operative administration of aprotinin during cardio-pulmonary bypass surgery.

A kit for use in the detection of an early biomarker of impaired renal status, the kit comprising:

• a. a means for acquiring a quantity of a urine sample;
• b. a media having affixed thereto a capture antibody capable of complexing with an early biomarker of impaired renal status, the early biomarker selected from the group consisting of aprotinin, alpha-1-microglobulin, alpha-1-acid-glycoprotein, microalbumin, and combinations thereof;
• and
• c. an assay for the detection of a complex of the early biomarker of impaired renal status and the capture antibody.