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OwnerAndrew Alaskainventor
Estimating the term…
Devices for expanded bed chromatography use inlet and outlet ports beneath a mesh that supports sorbent beads in a column. Placement of both ports beneath the mesh provides a horizontal “sweep flow” tangential to the mesh, to suppress the formation of particulate cakes on the lower surface of the mesh when liquids containing high particulate loads (such as cells or cell debris) are being processed. This design can be used with vibrators, hammering devices, intermittent reverse flow, or other means for disrupting the formation of particulate cakes or aggregates. Disrupters can also be used during elution, to accelerate the release of the valuable molecules from the sorbent. Initial tests indicate that these systems can efficiently handle heavily loaded liquids that would rapidly clog other systems previously known in the art.
A method of processing a liquid containing a mixture of desired target molecules and undesired particulates, comprising pumping said liquid into a column device containing a sorbent material that is retained within said column by a semi-permeable support component that is permeable to the liquid and undesired particulates but impermeable to the sorbent material, wherein the liquid containing the target molecules and particulates is pumped into a fluid flow compartment that is provided with an inlet and an outlet, wherein flow rates through each of said inlet and outlet can be adjusted in a manner that can establish fluid flow through said fluid flow compartment in a direction tangential to the semi-permeable support component.
A device for processing a liquid containing a mixture of desired target molecules and undesired particulates, comprising:
Dependencies are inferred from the claim text.