Flowering inhibition
AssigneesAgriculture Victoria Services PTY LTD, AgResearch Limited
InventorsIgor Kardailsky, Bruce Veit, Natasha Forester, Milan Gagic, Kim Richardson, Martin Faville
Life of this patent
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Abstract
The present invention relates to nucleic acids or nucleic acid fragments encoding amino acid sequences for proteins involved in the control of flowering in plants, and the use thereof for the modification of flowering, particularly inhibiting flowering. In particular, the present invention relates to nucleic acids or nucleic acid fragments encoding amino acid sequences of FLOWERING LOCUS T (FT), TERMINAL FLOWER (TFL), GIGANTEA (GI) and SHORT VEGETATIVE PHASE (SVP) polypeptides.
Claims23
1-30. (canceled)
A substantially purified or isolated nucleic acid or nucleic acid fragment from a ryegrass (Lolium) or fescue (Festuca) species encoding
- (1) a FLOWERING LOCUS T (FT) polypeptide or complementary or antisense to a sequences encoding FT, the presence of which inhibits flowering, and including a nucleotide sequence selected from the group consisting of (a) SEQ ID No 1, SEQ ID No 2 or SEQ ID No 3;
- (b) complements of the sequences recited in (a);
- (c) sequences antisense to the sequences recited in (a) and (b);
- (d) functionally active fragments and variants of the sequences recited in (a), (b) and (c);
- and (e) RNA sequences corresponding to the sequences recited in (a), (b), (c) and (d);
- (2) a TERMINAL FLOWER (TFL) polypeptide or complementary or antisense to a sequences encoding TFL, the presence of which inhibits flowering, and including a nucleotide sequence selected from the group consisting of (a) SEQ ID No 4;
- (b) the complement SEQ ID No 4;
- (c) sequences antisense to the sequences recited in (a) and (b);
- and (d) RNA sequences corresponding to the sequences recited in (a), (b), and (c);
- (3) a TERMINAL FLOWER (TFL) polypeptide or complementary or antisense to a sequences encoding TFL, the presence of which inhibits flowering, and including a nucleotide sequence selected from the group consisting of (a) SEQ ID No 5;
- (b) the complement SEQ ID No 5;
- (c) sequences antisense to the sequences recited in (a) and (b);
- (d) sequences having at least approximately 90% homology to the sequences recited in (a), (b) and (c);
- and (e) RNA sequences corresponding to the sequences recited in (a), (b), (c) and (d);
- (4) a GIGANTEA (GI) or SHORT VEGETATIVE PHASE (SVP) polypeptide or complementary or antisense to a sequences encoding GI or SVP, and including a nucleotide sequence selected from the group consisting of (a) SEQ ID No 6, SEQ ID No 7, SEQ ID No 8, SEQ ID No 10, SEQ ID No 11, or SEQ ID No 12;
- (b) complements of the sequences recited in (a);
- (c) sequences antisense to the sequences recited in (a) and (b);
- (d) functionally active fragments and variants of the sequences recited in (a), (b) and (c);
- and (e) RNA sequences corresponding to the sequences recited in (a), (b), (c) and (d);
- or
- (5) a SHORT VEGETATIVE PHASE (SVP) polypeptide or complementary or antisense to a sequences encoding SVP, and including a nucleotide sequence selected from the group consisting of (a) SEQ ID No 9;
- (b) complements of the sequences recited in (a);
- (c) sequences antisense to the sequences recited in (a) and (b);
- and (d) RNA sequences corresponding to the sequences recited in (a), (b) and (c).
A nucleic acid or nucleic acid fragment including an SNP according to claim 43, wherein said nucleic acid or nucleic acid fragment is from perennial ryegrass (Lolium perenne).
A substantially purified or isolated polypeptide from a ryegrass (Lolium) or fescue (Festuca) species, selected from the group consisting of
- (1) FT polypeptides including an amino acid sequence selected from the group consisting of sequences translated from the nucleotide sequences of SEQ ID No 1, SEQ ID No 2 or SEQ ID no 3 and functionally active fragments and variants thereof;
- (2) TFL polypeptides including an amino acid sequence selected from the group consisting of sequences translated from the nucleotide sequences of SEQ ID No 4 or SEQ ID No 5;
- (3) GI or SVP polypeptides including an amino acid sequence selected from the group consisting of (a) sequences translated from the nucleotide sequences of SEQ ID No 6, SEQ ID No 7, SEQ ID No 8, SEQ ID No 10, SEQ ID No 11, or SEQ ID No 12 and (b) functionally active fragments and variants of (a);
- and
- (4) SVP polypeptides including an amino acid sequence translated from the nucleotide sequence of SEQ ID No 9.
A polypeptide according to claim 45, wherein said polypeptide is from perennial ryegrass (Lolium perenne).
A substantially purified or isolated nucleic acid or nucleic acid fragment from a ryegrass (Lolium) or fescue (Festuca) species encoding a FLOWERING LOCUS T (FT) polypeptide, the presence of which inhibits flowering, and including a nucleotide sequence selected from the group consisting of (a) SEQ ID No 1, SEQ ID No 2 and SEQ ID No 3.
A substantially purified or isolated nucleic acid or nucleic acid fragment from a ryegrass (Lolium) or fescue (Festuca) species encoding a TERMINAL FLOWER (TFL) polypeptide, the presence of which inhibits flowering, and including the nucleotide sequence of SEQ ID No 4.
A substantially purified or isolated nucleic acid or nucleic acid fragment from a ryegrass (Lolium) or fescue (Festuca) species encoding a TERMINAL FLOWER (TFL) polypeptide, the presence of which inhibits flowering, and including the nucleotide sequence of SEQ ID No 5.
A substantially purified or isolated nucleic acid or nucleic acid fragment from a ryegrass (Lolium) or fescue (Festuca) species encoding a GIGANTEA (GI) or SHORT VEGETATIVE PHASE (SVP) and including a nucleotide sequence selected from the group consisting of SEQ ID No 6, SEQ ID No 7, SEQ ID No 8, SEQ ID No 10, SEQ ID No 11 and SEQ ID No 12.
A substantially purified or isolated nucleic acid or nucleic acid fragment from a ryegrass (Lolium) or fescue (Festuca) species encoding a SHORT VEGETATIVE PHASE (SVP) polypeptide and including the nucleotide sequence of SEQ ID No 9.
Dependencies are inferred from the claim text.