Flax (Linum usitatissimum I.) seed-specific promoters
AssigneeBioriginal Food & Science Corp.
InventorsXiao Qiu, Martin Truksa, Zhiyuan Hu
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Abstract
The present invention is directed to promoters of flax conlinin and ω-3 desaturase genes. The promoters guide high levels of the expression exclusively in flax developing seeds. This specific expression pattern concomitant with the biosynthesis of storage lipids and proteins make these promoters particularly useful for seed-specific modification of fatty acid and protein compositions in plant seeds.
Claims21
An isolated nucleic acid molecule which encodes a polypeptide having an activity of catalyzing the formation of a double bond, wherein said nucleic acid molecule comprises a nucleotide sequence selected from the group consisting of:
- a. a nucleotide sequence of LuFad3 from the genus Linum, or a complement thereof;
- b. a nucleotide sequence which is at least 60% identical to the entire nucleotide sequence of SEQ ID NO:7, or a complement thereof;
- c. a nucleotide sequence comprising a fragment of the nucleotide sequence of SEQ ID NO:7, or a complement thereof;
- d. a nucleotide sequence which encodes a polypeptide comprising an amino acid sequence that is at least 70% identical to the entire amino acid sequence of SEQ ID NO:8, or a complement thereof;
- e. a nucleotide sequence which encodes a fragment of at least 15 contiguous amino acid residues of SEQ ID NO:8, or a complement thereof;
- and
- f. a nucleotide sequence which encodes a naturally occurring allelic variant of a polypeptide comprising the amino acid sequence of SEQ ID NO:8, wherein the nucleic acid molecule hybridizes to a nucleic acid molecule comprising SEQ ID NO:7 under stringent conditions, or a complement thereof.
An isolated nucleic acid molecule, wherein said nucleic acid molecule comprises a nucleotide sequence selected from the group consisting of:
- a. a nucleotide sequence of Conlinin 1 or Conlinin 2 from the genus Linum, or a complement thereof;
- b. a nucleotide sequence which is at least 60% identical to the entire nucleotide sequence of SEQ ID NO:1 or 3, or a complement thereof;
- c. a nucleotide sequence comprising a fragment of the nucleotide sequence of SEQ ID NO:1 or 3, or a complement thereof;
- d. a nucleotide sequence which encodes a polypeptide comprising an amino acid sequence that is at least 60% identical to the entire amino acid sequence of SEQ ID NO:2 or 4, or a complement thereof;
- e. a nucleotide sequence which encodes a fragment of at least 15 contiguous amino acids of SEQ ID NO:2 or 4, or a complement thereof;
- and
- f. a nucleotide sequence which encodes a naturally occurring allelic variant of a polypeptide comprising the amino acid sequence of SEQ ID NO:2 or 4, wherein the nucleic acid molecule hybridizes to a nucleic acid molecule comprising SEQ ID NO:1 or 3 under stringent conditions, or a complement thereof.
A method of producing a polypeptide comprising culturing the host cell of claim 6 in an appropriate culture medium to, thereby, produce the polypeptide.
An isolated promoter selected from the group consisting of:
- a) a promoter comprising a nucleotide sequence isolated from Linum which is capable of directing gene expression in developing flax seeds;
- b) an isolated Linum Conlinin 1 promoter comprising the nucleotide sequence of SEQ ID NO:5, or a portion thereof;
- c) an isolated Linum Conlinin 2 promoter comprising the nucleotide sequence of SEQ ID NO:6, or a portion thereof;
- d) an isolated Linum LuFad 3 promoter comprising the nucleotide sequence of SEQ ID NO:
- 9, or a portion thereof;
- and
- e) an isolated Linum LuFad 3 promoter comprising the nucleotide sequence of SEQ ID NO:
- 10, or a portion thereof.
An isolated nucleic acid sequence that directs seed-specific expression in a plant comprising a nucleic acid sequence selected from the group consisting of:
- a) a nucleic acid sequence comprising the nucleotide sequence of SEQ ID NO:
- 5, 6, 9 or 10;
- b) a nucleic acid sequence that is complementary to the nucleotide sequence of SEQ ID NO:
- 5, 6, 9 or 10;
- and
- c) a nucleic acid sequence that is at least 60% identical to the entire nucleotide sequence of SEQ ID NO:
- 5, 6, 9 or 10;
The nucleic acid construct of 18, wherein the gene is selected from the group consisting of a conjugase, Δ4 desaturase, Δ5 desaturase, and Δ6 desaturase.
A promoter capable of directing gene expression in developing flax seeds operatively linked to a gene related to fatty acid biosythesis or lipid biosynthesis, wherein the promoter comprises at least 701 nucleotides of SEQ ID NO:5, 751 nucleotides of SEQ ID NO:5, 801 nucleotides of SEQ ID NO:5, 851 nucleotides of SEQ ID NO:5, 901 nucleotides of SEQ ID NO:5, 951 nucleotides of SEQ ID NO:5, 1,001 nucleotides of SEQ ID NO:5, or 1,118 nucleotides of SEQ ID NO:5.
A promoter capable of directing gene expression in developing flax seeds operatively linked to a gene related to fatty acid biosythesis or lipid biosynthesis, wherein the promoter comprises at least 597 nucleotides of SEQ ID NO:6, 647 nucleotides of SEQ ID NO:6, 697 nucleotides of SEQ ID NO:6, 746 nucleotides of SEQ ID NO:6, 797 nucleotides of SEQ ID NO:6, 846 nucleotides of SEQ ID NO:6, 897 nucleotides of SEQ ID NO:6, 947 nucleotides of SEQ ID NO:6, 997 nucleotides of SEQ ID NO:6, or 1,014 nucleotides of SEQ ID NO:6.
Dependencies are inferred from the claim text.