# US 2007/0192898 A1 · Proteins inducing multiple resistance of plants to phytopathogens and pests > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2007/0192898 A1 - Document ID: US20070192898A1 - Status: Published application - Kind: A1 - Application number: 10/583,066 - Filed: 2004-12-17 - Published: 2007-08-16 - Earliest claimed: 2003-12-22 - Inventors: Vitaly Dzhavakhia; Alexei Filippov; Konstantin Skryabin; Tatiana Voinova; Maria Kouznetsova; Olga Shulga; Daria Shumilina; Ksenia Kromina; Mikhail Pridanniko; Natalia Battchikova; Timo Korpela - Web page: https://patentgrep.com/patent/US20070192898A1 ## CPC - C12N 15/8283: {for virus resistance} - A01N 63/50: Isolated enzymes; Isolated proteins (peptides A01N37/46) - C12N 15/8281: {for bacterial resistance} - C12N 15/8285: {for nematode resistance} - Y02A 40/146: Genetically Modified [GMO] plants, e.g. transgenic plants - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS - Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE ## Abstract The object of the invention is a protein, termed MF3, or a functional derivative thereof with a novel structure that surprisingly can induce multiple resistance in plants toward a variety of viral and microbial infections and against pests. The invention also concerns an isolated DNA sequence encoding MF3 protein, as such, or as a part of any DNA sequence, or a fragment thereof, or DNA sequences which have degenerate codons with respect to the DNA sequence defined above. The invention also concerns a method of isolating and purifying the protein MF3 from bacterial cells expressing the said protein and its use as a plant protectant with or without carrier agent. Furthermore, the invention concerns a method of obtaining transgenic plants expressing said protein. A further object of the invention is the use of the protein, or of compositions containing the same, as a plant protectant, biopesticide for inducing resistance of plants to viral, microbial phytopathogens and pests. ## Claims ### Claim 1 A bioactive polypeptide, MF3, with a primary structure depicted in SEQ ID NO:1, an active fragment of MF3, or any functional derivative of MF3, said polypeptide, active fragment or functional derivative being capable of effecting a resistance of a plant against microbial diseases and/or against attack of plant parasites. ### Claim 2 (depends on 1) An isolated DNA sequence depicted in SEQ ID:2, or fragment thereof, encoding a functionally active MF3 or its active fragment according to claim 1, wherein said DNA fragment may contain degenerate codons. ### Claim 3 (depends on 1) A method of acquiring resistance of a plant against microbes and/or plant parasites by introducing the bioactive polypeptide MF3 of claim 1, or an active fragment, or a functional derivative thereof into plants mechanically or by means of carrier molecules. ### Claim 4 (depends on 3) The method according to claim 3, wherein the carrier is chitosan. ### Claim 5 (depends on 2) A vector comprising the DNA according to claim 2. ### Claim 6 (depends on 5) A transgenic plant or plant cell culture comprising a vector according to claim 5. ### Claim 7 (depends on 5) A host cell stably transformed or transfected with a vector of claim 5. ### Claim 8 (depends on 1) A plant protectant composition comprising isolated bioactive polypeptide MF3, an isolated active fragment of MF3 or any isolated functional derivative of MF3 of claim 1. ### Claim 9 (depends on 1) The active fragment of MF3 according to claim 1, wherein the amino acid sequence consists of SEQ ID:3 or SEQ ID:4. ### Claim 10 (depends on 1) A method of isolating and purifying the polypeptide of claim 1 from bacterial cells expressing said polypeptide, the method comprising the steps: a) cultivating a microbial producer strain and extracting cells with a buffer solution at an elevated temperature; b) precipitating a crude MF3 polypeptide at low temperature with a precipitant; c) fractionating re-dissolved precipitate by an anion exchange chromatography column and collecting fractions with anti-microbial or anti-insect activities; d) performing polyacrylamide gel electrophoresis of the polypeptide fractions with anti-microbial, anti-nematode, or anti-insect activities; e) recovering the protein eluted from the gel of step d. ### Claim 11 (depends on 8) A method to protect plants or plant cell cultures from microbial diseases or pests by applying the protectant composition of claim 8. ### Claim 12 (depends on 11) The method according to claim 11, wherein the plants or plant cell cultures are protected from diseases caused by a microbe selected from the group consisting of Phytophtora infestans, Erwinia carotovora, Pyricularia oryzae, Fusarium cumorum, Septoria nodorum, Tobacco Mosaic Virus, Potato Virus X, and Potato Virus Y. ### Claim 13 (depends on 11) The method according to claim 11, wherein the plants are protected from potato cyst nematodes. ### Claim 14 (depends on 6) The transgenic plant or plant cell culture of claim 6, wherein the transgenic plant or cell culture expresses increased resistance against a disease caused by a microbe selected from the group consisting of Phytophtora infestans, Erwinia carotovora, Pyricularia oryzae, Fusarium cumorum, Septoria nodorum, Tobacco Mosaic Virus, Potato Virus X, and Potato Virus Y. ### Claim 15 (depends on 6) The transgenic plant or plant cell culture of claim 6, wherein the transgenic plant or cell culture expresses increased resistance against potato cyst nematodes.