Soybean Root-Preferred, Nematode-Inducible Promoter and Methods of Use
AssigneePioneer Hi-Bred International, Inc.
InventorsXu Hu, Guihua Lu
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Abstract
The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a root-preferred and inducible promoter for the gene encoding a soybean ripening-related protein. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.
Claims24
An isolated nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of:
- a) a nucleotide sequence comprising the sequence set forth in SEQ ID NOs:
- 1-4 or a full-length complement thereof;
- b) a nucleotide sequence comprising the plant promoter sequence of the plasmid deposited as Patent Deposit No. PTA-6952, or a full-length complement thereof;
- c) a nucleotide sequence comprising a fragment of a) or b), wherein said sequence initiates transcription in a plant cell;
- and
- d) a nucleotide sequence comprising a sequence having at least 85% sequence identity to the sequence set forth in SEQ ID NOs:
- 1-4, wherein said sequence initiates transcription in the plant cell.
A method for expressing a nucleotide sequence in a plant, said method comprising introducing into a plant a DNA construct, said DNA construct comprising a promoter and operably linked to said promoter a heterologous nucleotide sequence of interest, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
- a) a nucleotide sequence comprising the sequence set forth in SEQ ID NOs:
- 1-4;
- b) a nucleotide sequence comprising the plant promoter sequence of the plasmid designated as Patent Deposit No. PTA-6952;
- c) a nucleotide sequence comprising a fragment of a) or b), wherein said nucleotide sequence initiates transcription in said plant;
- and
- d) a nucleotide sequence comprising a sequence having at least 85% sequence identity to the sequence set forth in SEQ ID NOs:
- 1-4, wherein said nucleotide sequence initiates transcription in a plant cell.
A method for expressing a nucleotide sequence in a plant cell, said method comprising introducing into a plant cell a DNA construct comprising a promoter operably linked to a heterologous nucleotide sequence of interest, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
- a) a nucleotide sequence comprising the sequence set forth in SEQ ID NOs:
- 1-4;
- b) a nucleotide sequence comprising the plant promoter sequence of the plasmid designated as Patent Deposit No. PTA-6952;
- c) a nucleotide sequence comprising a fragment of a) or b), wherein said nucleotide sequence initiates transcription in said plant;
- and
- d) a nucleotide sequence comprising a sequence having at least 85% sequence identity to the sequence set forth in SEQ ID NOs:
- 1-4, wherein said nucleotide sequence initiates transcription in a plant cell.
A method for selectively expressing a nucleotide sequence in a plant root, said method comprising introducing into a plant cell a DNA construct, and regenerating a transformed plant from said plant cell, said DNA construct comprising a promoter and a heterologous nucleotide sequence operably linked to said promoter, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
- a) a nucleotide sequence comprising the sequence set forth in SEQ ID NOs:
- 1-4;
- b) a nucleotide sequence comprising the plant promoter sequence of the plasmid designated as Patent Deposit No. PTA-6952;
- c) a nucleotide sequence comprising a fragment of a) or b), wherein said nucleotide sequence initiates transcription in said plant;
- and
- d) a nucleotide sequence comprising a sequence having at least 85% sequence identity to the sequence set forth in SEQ ID NOs:
- 1-4, wherein said nucleotide sequence initiates transcription in a plant cell.
Dependencies are inferred from the claim text.