Method for crystallization of proteins using polysaccharides
AssigneeMacrocrystal Oy
InventorsKalevi Visuri, Sinikka Uotila, Katja Palmunen
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Abstract
The objective of the invention is a crystallizing method for macromolecules, especially proteins and polypeptides, in which selected polysaccharides of biological origin, such as alginate, pectin, dextrin or chitosan and hydrolysates thereof, are used as reagents. Sedimentation of the crystals can be prevented and thus the uniformity of the product contributed with the method. The method can be used to prepare new crystal forms of the polypeptide and to improve the stability of crystals.
Claims11
A method for crystallization of proteins and peptides, whererin (a) a protein solution or a peptide solution, in which the solvent is water, and (b) a polymer solution, in which alginate or dextrin or chitosan or pectin or hydrolysate of any above mentioned polymer or a mixture of any above mentioned polymer is dissolved in water, are prepared and that the prepared solutions (a) and (b) are mixed together and that after the combining the protein or the peptide crystallizes permanently.
Dependencies are inferred from the claim text.