Polysaccharide compositions and methods of producing, screening, and formulating polysaccharide compositions
AssigneeSolazyme, Inc.
InventorsHarrison F. Dillon, Aravind Somanchi, Anwar Zaman, Kamalesh Rao, Jonathan Wolfson
Life of this patent
Estimating the term…
Abstract
Provided herein are polysaccharide compositions and methods of culturing microalgae to produce polysaccharides. Also provided are methods of using polysaccharides for applications such as reducing cholesterol in mammals, inactivating viruses, stabilizing foods, and other uses. Also provided are transgenic algae capable of utilizing fixed carbon sources for energy.
Claims192
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
A polysaccharide produced from a cell of the genus Porphyridium, comprising xylose, glucose, and galactose wherein the molar amount of glucose in the polysaccharide is at least 65% of the molar amount of galactose.
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
A method of producing a polysaccharide comprising culturing a microalgae cell in the presence of at least 0.01 micromolar of a compound, wherein the compound is incorporated into the polysaccharide produced by the cell.
(canceled)
(canceled)
(canceled)
A cell of the genus Porphyridum comprising an exogenous gene that encodes a carbohydrate transporter protein.
A method of trophically converting a cell of the genus Porphyridium comprising:
- a. providing a nucleic acid encoding a carbohydrate transporter protein;
- b. transforming the cell with the nucleic acid;
- and
- c. selecting for the ability to undergo cell division
- i. in the absence of light;
- and
- ii. in the presence of a carbohydrate that is transported by the carbohydrate transporter protein.
An expression vector comprising a nucleic acid sequence encoding a carbohydrate transporter protein, wherein the nucleic acid has at least 60% nucleotide identity with one or more of SEQ ID NOs:
- 21, 23 or 25.
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
A method of producing a glycopolymer comprising
- a. providing a transgenic cell containing a recombinant gene encoding a monosaccharide transporter;
- and
- b. culturing the cell in the presence of at least one monosaccharide, wherein the monosaccharide is transported by the transporter into the cell and is incorporated into the glycopolymer.
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
Dependencies are inferred from the claim text.