US 2007/0191280 A1 · High Purity Lipopeptides

Abstract

The invention discloses highly purified daptomycin and to pharmaceutical compositions comprising this compound. The invention discloses a method of purifying daptomycin comprising the sequential steps of anion exchange chromatography, hydrophobic interaction chromatography and anion exchange chromatography. The invention also discloses a method of purifying daptomycin by modified buffer enhanced anion exchange chromatography. The invention also discloses an improved method for producing daptomycin by fermentation of Streptomyces roseosporus. The invention also discloses high pressure liquid chromatography methods for analysis of daptomycin purity. The invention also discloses lipopeptide micelles and methods of making the micelles. The invention also discloses methods of using lipopeptide micelles for purifying lipopeptide antibiotics, such as daptomycin. The invention also discloses using lipopeptide micelles therapeutically.

Claims53

Dependent claims

A composition comprising

(a) essentially pure daptomycin,
(b) daptomycin that is substantially free of anhydro-daptomycin and substantially free of β-isomer of daptomycin,
(c) daptomycin that is essentially free of anhydro-daptomycin and substantially free of β-isomer of daptomycin,
(d) daptomycin that is free of anhydro-daptomycin and substantially free of β-isomer of daptomycin,
(e) daptomycin that is substantially free of each of impurities 1 to 14 defined by peaks 1-14 shown in FIG. 12,
(f) daptomycin that is essentially free of each of impurities 1 to 14 defined by peaks 1-14 shown in FIG. 12, or
(g) substantially pure daptomycin.

4 more elements

Dependencies are inferred from the claim text.

Facts

Application

11/739,180Filed 24 Apr 2007

Priority

29 Dec 2003

Published

16 Aug 2007

Term ends

Not estimatedThe source records don’t support an estimate.

Classification14 codes

A61K 38/12
Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof: cyclic peptides , e.g. bacitracins; Polymyxins; Gramicidins…
A61K 38/10
Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof: peptides having 12 to 20 amino acids
A61P 29/00
Non-central analgesic, antipyretic or antiinflammatory agents
A61P 31/00
Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics

High Purity Lipopeptides

Life of this patent

High Purity Lipopeptides

Life of this patent

Abstract

Claims53