Proteinaceous pharmaceuticals and uses thereof
OwnerWillem Stemmer, Volker Schellenberger +2inventors
InventorsWillem Stemmer, Volker Schellenberger, Martin Bader, Michael Scholle
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Abstract
The present invention provides cysteine-containing scaffolds and/or proteins, expression vectors, host cell and display systems harboring and/or expressing such cysteine-containing products. The present invention also provides methods of designing libraries of such products, methods of screening such libraries to yield entities exhibiting binding specificities towards a target molecule. Further provided by the invention are pharmaceutical compositions comprising the cysteine-containing products of the present invention.
Claims24
A non-naturally occurring cysteine (C)-containing protein comprising a polypeptide having no more than 35 amino acids, in which
- at least 10% of the amino acids in the polypeptide are cysteines,
- at least two disulfide bonds are formed by pairing intra-scaffold cysteines, and wherein said pairing yields a complexity index greater than 3.
A non-naturally occurring cysteine (C)-containing protein comprising a polypeptide having no more than about 60 amino acids, in which
- at least 10% of the amino acids in the polypeptide are cysteines,
- at least four disulfide bonds are formed by pairing cysteines contained in the polypeptide, and wherein said pairing yields a complexity index greater than 4.
The non-naturally occurring cysteine (C)-containing protein exhibiting binding specificity towards a target distinct from the native target of the corresponding naturally-occurring cysteine (C)-containing protein or scaffold.
A non-natural protein containing a single domain of 20-60 amino acids which has 3 or more disulfides and binds to a human serum-exposed protein, and wherein said protein has less than 5% aliphatic amino acids.
A non-naturally occurring protein containing a single domain of 20-60 amino acids which has 3 or more disulfides and binds to a human serum-exposed protein, wherein said protein has a score in the T-Epitope program that is less than 90% of the average for proteins in the database.
Dependencies are inferred from the claim text.