Apolipoprotein biopolymer markers indicative of insulin resistance
AssigneeNanogen, Inc.
InventorsGeorge Jackowski, John Marshall
Life of this patent
Estimating the term…
Abstract
The instant invention involves the use of a combination of preparatory steps in conjunction with mass spectroscopy and time-of-flight detection procedures to maximize the diversity of biopolymers which are verifiable within a particular sample. The cohort of biopolymers verified within such a sample is then viewed with reference to their ability to evidence at least one particular disease state; thereby enabling a diagnostician to gain the ability to characterize either the presence or absence of said at least one disease state relative to recognition of the presence and/or the absence of said biopolymer, predict disease risk assessment, and develop therapeutic avenues against said disease.
Claims10
An isolated biopolymer marker consisting of SEQ ID NO:1 indicative of insulin resistance.
2-38. (canceled)
A method for determining presence of an isolated biopolymer marker indicative of insulin resistance consisting of SEQ ID NO:1 from-a mass spectrometric analysis of a sample obtained from a patient comprising:
- (a) preparing said sample for a mass spectrometric analysis by subjecting proteins contained in said sample to chromatography, electrophoresis and enzymatic digestion;
- (b) conducting mass spectrometric analysis on said sample as prepared in step (a) to obtain mass spectral profiles of peptides contained therein;
- (c) comparing a reference mass spectral profile characteristic of a isolated biopolymer marker consisting of SEQ ID NO:1 having an ion peak at about 1311.7061 daltons to mass spectral profiles of peptides obtained from said sample;
- and
- (d) matching said reference mass spectral profile to a mass spectral profile of a peptide obtained from said sample by identifying said ion peak at about 1311.7061 daltons:
- wherein a match confirms presence of a biopolymer marker indicative of insulin resistance consisting of SEQ ID NO:1.
(canceled)
A kit for determining presence of an isolated biopolymer marker indicative of insulin resistance consisting of SEQ ID NO:1 comprising:
- (a) a peptide consisting of SEQ ID NO:1 and (b) an antibody that binds to said peptide in a sample obtained from a patient.
Dependencies are inferred from the claim text.