Pulsatile perfusion extraction method for non-embryonic pluripotent stem cells
AssigneeHemacell Perfusion
InventorFred Gage
Life of this patent
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Abstract
A method for extracting stem cells from a non-embryonic stem cell source, including providing a non-embryonic stem cell source including stem cells; perfusing the non-embryonic stem cell source with a pulsatile flow of a perfusion solution to produce a perfusate including stem cells and a perfused non-embryonic stem cell source; and isolating the stem cells from the perfusate to produce isolated stem cells, is provided. Also provided is a non-embryonic stem cell line derived from a non-embryonic stem cell obtained using the pulsatile perfusion extraction method.
Claims20
A method for extracting stem cells from a non-embryonic stem cell source, comprising:
- providing a non-embryonic stem cell source comprising stem cells;
- perfusing the non-embryonic stem cell source with a pulsatile flow of a perfusion solution to produce a perfusate comprising stem cells and a perfused non-embryonic stem cell source;
- and
- isolating the stem cells from the perfusate to produce isolated stem cells.
Dependencies are inferred from the claim text.